Exploration of the blocking effect of Fab fragment of IgG autoantibody on erythrocyte autoantigens
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 9
Abstract
Objective To use Fab segments of autoantibodies to bind to autoantigens on red blood cells in order to block the binding of intact autoantibodies to the autoantigens. Methods The pure autoantibodies were obtained from the serum of patients by absorption and elution test, then Fab segments were obtained by hydrolyzing IgG autoantibodies at 37℃ overnight with papain enzyme. The Fab segments were used to bind the corresponding autoantigens specifically, thus blocking the binding of intact autoantibodies to autoantigens. Results The pure autoantibodies obtained through absorption and elution test were all hydrolyzed by papain to generate Fab fragments. When mixed with intact autoantibodies in their respective elution solutions, these Fab fragments were able to block the binding of intact autoantibodies to red blood cell autoantigens. Among the three samples, there was a mutual blocking effect between sample 1 and sample 2, while no mutual blocking effect was observed between sample 2 and sample 3. Conclusion Fab segment of autoantibody can eliminate the agglutination of intact autoantibodies and erythrocyte autoantigens, and can be applied to clinical practice to solve clinical problems.
Authors and Affiliations
Haitao HUANG, Xuehong WEN
Keywords
Related Articles
- EP ID EP748059
- DOI 10.13303/j.cjbt.issn.1004549x.2024.09.020
- Views 36
- Downloads 0
Effects of intraoperative blood salvage on red blood cells
Intraoperative blood salvage (IBS) is a life support technology that uses a series of artificial devices to treat the recycled blood during surgery, which then is transfused back into the patients. Currently, it′s the ke...
Blood transfusion adverse event management system: construction and application
Objective To establish a blood transfusion adverse event management system and apply it to adverse events management, so as to enhance the ability to identify and process reports as well as prevent adverse events. Method...
Efficacy of three plasma exchange methods in improving renal insufficiency after kidney transplantation and the induction of plasma exchange related adverse reactions: a comparative study
Objective To compare the efficacy of double filtration plasmapheresis(DFPP), centrifugal therapeutic plasma exchange(cTPE) and centrifugation-filtration plasmapheresis(CFPP) in improving renal insufficiency after kidney...
Study on the extensibility of platelet donor gene database in Shaanxi
Objective To analyze the commonality and characteristics between voluntary blood donors and hematopoietic stem cell donors in this region, and explore the potential for integration and development between China Marrow Do...
Application and evaluation of human antibodies in ABO grouping
Objective To evaluate the application of monoclonal typing reagents and human anti-A/B antibodies for absorption-elution test in ABO grouping. Methods The specificity of monoclonal typing reagents and human anti-A/B anti...