Exploring the industrially important extracellular enzymes from different nematode-trapping fungi
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 2
Abstract
Nematophagous fungi are potential bio-control agent against the plant and animal parasitic nematodes. The extracellular hydrolytic enzymes are one of the important virulence factors for these fungi. In the present study, we investigated industrially important extracellular enzymes from two different genera of nematode-trapping fungi. Duddingtonia flagrans produced the highest amount of amylase (261 U/mL/min) and xylanase (76mU/mL/min) compared to Arthrobotrys conoides (186 and 39 U/mL/min) while it was reversed in case of cellulase, highest (214 U/mL/min) in A. conoides. Protease, chitinase and collagenase activity (236, 19.83 and 2.46 U/mL/min) was higher during induction in D. flagrans RPAN-10 as compared to A. conoides (118.6, 8.3 and 1.2 U/mL/min). D. flagrans RPAN-10 showed 3.14 fold and A. conoides 2.37 fold change in protease activity when induced with nematodes. However, there was no apparent difference in control and induced fungus for chitinase and collagenase. In summary, D. flagrans RPAN-10 was found to be a suitable candidate for further exploring the protease, amylase, chitinase and xylanase while A. conoides is suitable for cellulase production for further application. However, further research is required in order to optimization of growth conditions as well as suitable nutrients in order for large scale production for industrial applications.
Authors and Affiliations
Anju Kunjadia, Nikita Darji, Babulal Patel, Ramesh Pandit
Keywords
Related Articles
- EP ID EP546133
- DOI -
- Views 98
- Downloads 0
Molecular detection and characterization of Canine distemper virus from domestic dogs (Canis familiaris) in Quezon City, Philippines
Canine distemper (CD) is a highly infectious viral disease affecting all terrestrial carnivores worldwide. In spite of the availability of commercial modified live vaccines, several studies have reported the occurrence...
Evaluation of phytochemicals, antioxidants and anti-inflammatory activity of fresh leaf and flower extracts of Tagetes erecta L. grown in Kalaburagi
Tagetes erecta L. (Asteraceae) is used to treat epileptic fits (Ayurveda), fever, wounds and microbial infections of livestock’s in folklore medicine of Kalaburagi district. As little work has been carried out on fre...
Environmental situation of Karla lake Maval, MS, India
Karla is a bygone lake in Shankar temple. The lake supports biodiversity, aquaculture and is important as recreation centre for the locals. Measures are undertaken for cleaning and protection of this lake. The present p...
A study of effect of Physico-chemical parameters on biodiversity of Diatoms special Reference to Navicula in river Narmada at Harda, M.P. India
Present paper represents the seasonal variation of physico-chemical parameters and its correlation to species of diatom Navicula. Navicula is a dominant genus of diatom. Seasonal average value of physico-chemical paramet...
A new species of cestode Lytocestus paithanensis (Lytocestus Cohn, 1908) from Clarias batrachus at Paithan, MS, India
The present communication deals with description of new species Lytocestus paithanesis n.sp. is reported from Clarias batrachus at paithan Dist. Aurangabad. It difference from all known species of the genus, with the cha...