Expression analysis of three phenylpropanoid-related genes during Musa spp.- Mycosphaerella fijiensis interaction and in response to ethephon
Journal Title: Biotecnología Vegetal - Year 2010, Vol 10, Issue 4
Abstract
Phenylpropanoid pathway is one of the best-studied, most engineered and frequently induced by pathogens or pathogen elicitors. Patterns of expression of chalcone synthase (CHS), flavonoid 3’5' hydroxylase-like (F3’5’H-like) and isoflavone reductase-like (IFR-like), were analyzed in ‘Calcutta 4’ (resistant) and ‘Grande naine’ (susceptible) plants at 0, 6 and 12 days after artificial inoculation with M. fijiensis and after treatment with 500 ìM ethephon. Reverse transcriptase-polymerase chain reaction (RT- PCR) technique with specific primers was used for this purpose. In the face of fungal challenge F3’5’H-like and IFR-like genes had constitutive expression while CHS gene was induced (in a more significant way in ‘Calcutta 4’), in the time range tested for both genotypes. The ethylene producer ethephon induced IFR-like gene in the resistant genotype and in the susceptible one the F3’5’H-like gene. These results will let to continue a deeper study in phenylpropanoid role during Musa-M. fijiensis interaction.
Authors and Affiliations
Milady Mendoza-Rodríguez , Orelvis Portal , Bárbara Ocaña , Mayra Acosta-Suárez , Berkis Roque , Monica Höfte , Elio Jiménez
Keywords
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