EXPRESSION OF ADAMTS10 IN MALE REPRODUCTIVE TRACT OF BUFFALOES (Bubalus bubalis)
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2018, Vol 6, Issue 5
Abstract
Large number of proteases have been identified in different parts of the male reproductive tract which play a crucial role during testicular development, epididymal sperm maturation, and sperm-oocyte fusion. The ADAMTS10 is a member of metalloproteinase family, the expression of which is important in sperm-oocyte interaction at the time of fertilization in mouse. In this species, the expression of ADAMTS10 has been demonstrated in testis, and on surface of spermatozoa from caput, corpus and cauda epididymis. Whether ADAMTS10 plays any role in male fertility of any of the domestic ruminant species including buffaloes is not known so far. Thus, a study was conducted to detect the expression of ADAMTS10 in testis, different parts of the epididymis and ejaculated spermatozoa of water buffalo (Bubalus bubalis) using real-time PCR, Western blot, and indirect immunofluorescence techniques. The highest expression of ADAMTS10 transcript was observed in corpus epididymis, followed by cauda, caput and testis. The Western blot detection of ADAMTS10 using the heterologous antibody has demonstrated 31-, 44-, 50-, 65 kDa immune-reactive protein bands from testis and 82 kDa protein from both testes and corpus epididymis and a major 36 kDa protein from ejaculated spermatozoa. The indirect immunofluorescence study has demonstrated high fluorescence signal in post-acrosome and the middle piece region of the ejaculated spermatozoa. Thus the differential expression pattern of ADAMTS10 in different parts of male reproductive tract of buffalo indicates their role in sperm maturation process. However, specific role of ADAMTS10 in buffalo male reproduction can only be ascertained after further studies.
Authors and Affiliations
Vijayasaraswathy S. Gurupriya, Sudhir C. Roy, Pradeep K. Javvaji, Arindam Dhali, Sharanabasav Badami, Fathima Rahim, Bannur C. Divyashree, Arya P. Panda
Keywords
Related Articles
- EP ID EP593080
- DOI 10.18006/2018.6(5).800.807
- Views 62
- Downloads 0
SELECTION AND IDENTIFICATION OF A NOVEL THERMOTOLERANT Kluyveromyces marxianus STRAIN FOR VALORIZATION OF LACTOSE WASTE INTO ETHANOL
Sustainable production of ethanol as alternative renewable fuels, there is a need for a yeast strain which can convert the non-food substrate into ethanol economically. Extensive screening program for such yeast for bioe...
Reorienting dairy extension to meet changing needs of smallholder dairy production system
In the context of neo liberal policies Indian smallholder dairy farming is undergoing changes in milk processing, marketing, livestock services delivery, changing profile of producers and interaction between crop and dai...
ASSOCIATION OF THE METABOLIC SYNDROME AND VITAMIN D RECEPTOR GENE POLYMORPHISMS: A CROSS SECTIONAL STUDY
The Vitamin D Receptor gene (VDR) expressed in many tissues and are able to modulate the expression of several other genes. The purpose of this study was to assess the association between the metabolic syndrome (MetSyn)...
AGRO-MORPHOLOGICAL DIVERSITY OF EXOTIC TARO VARIETIES (Colocasia esculenta L. Schott) INTRODUCED IN BURKINA FASO
Aim of present study was to describe the agro-morphological characterization of fifty (50) exotic varieties of taro introduced in Burkina Faso. Further agronomic performances and genetic parameters were also evaluated in...
Do Marketing Objectives affect Marketing Efficiency? A Case Study of Dates marketing in Saudi Arabia
Saudi Arabia has dates as the most important agriculture crop, from both production and area harvested sides, its area was 155 thousand hectares and production was 991 thousand tons in 2010. Even that, the post productio...