Expression of HSP 70 and Mu Opioid Receptors Decrease Pain on Wet Cupping Therapy
Journal Title: Jurnal Ners - Year 2016, Vol 11, Issue 1
Abstract
Introduction: Wet cupping therapy widely used to reduce the pain, but the mechanisms of pain reduction on wet cuppig therapy is unclear. Stretching and punctures on wet cupping therapy cause stress on the cell. Cells that are stressed express HSP 70 and mu opioid receptors. This study aims to prove the correlation increased expression of HSP 70 and an increase in post-mu opioid receptor wet cupping therapy. Methods: The study is an experimental research design with randomized control group post-test only design. Twenty-four rats type Wistar (Rattus norvegicus) were randomly divided into three groups, each consisting of eight mice are negative control group (normal mice), group induction of pain with Complete Freund's Adjuvant (CFA) alone (positive control) and group by induction of pain and bekam basah therapy (10 puncture and negative pressure: - 200 mmHg, 5 minutes). Samples were taken from the skin and immunohistochemical examination with the monoclonal antibody anti mu opioid receptors. The reaction time pain threshold measured using a hot-plate. Data were analyzed by oneway ANOVA and Pearson Correlation using SPSS version 17. Results: This study shows that there is an increased expression of HSP 70 (P <0.05) compared to the positive control group (p <0.05) and increased expression of mu opioid receptors ( p <0.05) compared to the positive control group (p <0.05). There is an increased pain threshold reaction time (p <0.05) compared to the positive controls (11.78 ± 3.56). There is a correlation between an increased expression of HSP 70 and an increase in mu opioid receptor (β = 0893; p = 0.000) and there was a correlation between the increase in mu opioid receptor with a reaction time of pain threshold (β = 0713; p = 0.002). Discussion: Wet cupping therapy increase HSP 70 expression, opioid mu receptor, and decrease pain level.
Authors and Affiliations
Imam Subadi, Hening Laswati, Harjanto JM
Keywords
Related Articles
- EP ID EP199805
- DOI 10.20473/jn.V11I12016.34-39
- Views 117
- Downloads 0
Oxytocin Level and Breast Engorgement in Primipara Mother with Postpartum Blues
Introduction: Postpartum is a great moment for most the woman, but for some of them is a frightening moment. Postpartum blues makes woman avoiding their baby. Whereas lactation process needs to be done in early postpartu...
Development of Self Regulated Learning Model in Studying Nursing (SRLSN) to Improve Student Learning Competence
Introduction: New students at the college have to adjust to the learning process in a way more independent, not dependent on the lecturer, and self-regulation in learning. The purpose of this study is to develop a model...
Prediction of Mental Disorders Deprived by Family
Introduction: The family was the place and the fi rst to meet the basic needs of human beings, has fi ve tasks in health.The inability of the family perform its tasks will be a problem in a family member suffering from a...
Brain Gym Improves Cognitive Function for Elderly
Introduction: The degradation of cognitive function present early dementia in elderly. Brain gym is one of the alternative implementation to improve the cognitive function of elderly. The objective of this study was to a...
Improving Performance of Nursing Documentation Based on Knowledge Management Through SECI Concept Model’s
Introduction: Documentation of nursing care in Kalianget RSI in 2011 was totaled average 58,1% and in 2012 achieve was still low. According the lowest component was nursing diagnosis. This research aims to improve the pe...