Expression of MyoD in the DMBA-Treated Hamster Pouches Following Thymoquinone Injection.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 2
Abstract
Aim of study: To follow the sequential expression of Myo D in the DMBA-painted hamster buccal pouches, following early thymoquinone intraperitoneal injections. Material and Methods: Eighty male golden Syrian hamsters were divided into 3 groups: Group I: (10 animals) served as negative control, 5 hamsters were euthanized before starting the experiment, and the other 5 at end of the experiment. Group II: (10 animals) positive control (the left buccal pouches were painted with the carcinogen 0.5% DMBA 3/wk / 6 weeks), five hamsters were euthanized at second day of last painting and 5 hamsters 2 weeks later. Group III: (60 animals) thymoquinone (TQ)-treated group: were painted with DMBA, as in group II, then subdivided into two equal subgroups: IIIa: were given one i.p. injection of TQ (0.1 mg/kg). IIIb: were given two i.p. injections of TQ every other day. Blood samples were withdrawn for evaluation of TNF-α level before euthanization. Five animals, from the TQ-treated groups, were euthanized at 4, 24, 48 hrs, one week and two weeks after the last injection. All buccal pouches were surgically excised, fixed, and processed for H&E, and Myo D immunohistochemical (IHC) stains. Results: The results showed significant elevation of serum TNF-α after 6 weeks of DMBA as compared to the negative control group, and higher significant elevation following one i.p. injection of TQ as compared to all groups. Two i.p. injections of TQ resulted in significant elevation from the second day, then declined to near the DMBA-only group level. Clinically, after two weeks of 2 TQ i.p injections, elongation of the DMBA-painted pouches, was achieved to near normal control pouches’ length. IHC results showed Myo D expression was seen in perivascular mononuclear cells at areas of increasing muscle fibers’ bulk, in the TQ- treated groups, from day 2 of injections. These cells could be mesenchymal stem cells or pericytes. It was also expressed in the lower two thirds of the hyperplastic epithelium, near the distal necrotic side, as well as the nuclei of the bulk mature muscle fibers, from the first to the second week of injections. Conclusion: One and two ip injections with thymoquinone had promising effect to induce regeneration of the striated muscle layer independent on TNF-α or the muscle satellite cells, but due to other non-myogenic cell precursors.
Authors and Affiliations
Haethm M. Algharyni, Wafaa HH. El- Hossary, Ahmed MM. Korraah, Magda MA Hassan
Keywords
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- DOI 10.9790/0853-1802043540.
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