Expression of p16INK4A Protein in Cervical Intraepithelial Neoplasia and Invasive Carcinoma of Uterine Cervix
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 9
Abstract
Introduction: Cervical cancer still ranks as the most frequent cancer among women in India. Infection with Human Papilloma Virus (HPV) results in precancerous lesions and invasive cervical cancer. Interaction of HPV oncogenic proteins with cellular regulatory proteins leads to upregulation of p16INK4A, a cyclin dependent kinase inhibitor. Therefore, p16INK4A overexpression is a surrogate biomarker of HPV infection making it useful in evaluating HPV associated premalignant and malignant lesions of gynaecological tract. Aim: This study was conducted to evaluate the usefulness of p16INK4A as a biomarker in dysplastic and malignant alteration of cervical epithelium. Materials and Methods: A total of 57 specimens diagnosed histopathologically as cervical dysplasia or malignancy were included in this prospective study of two year duration. Immunohistochemical (IHC) study of p16INK4A expression was performed on normal cervical tissue samples, Cervical Intraepithelial Neoplasia (CIN) 1, CIN 2, CIN 3, squamous cell carcinoma, adenocarcinoma. Expression was scored by two methods. One was simple positive versus negative p16INK4A staining and the other method was a semi-quantitative IHC scoring (0 to 8 points) according to the intensity of staining and the proportion of stained cells. The collected data was entered in excel sheet and analysed using Statistical Package For Social Science (SPSS) 16.0 software and the descriptive statistics, Chi-square test. The p-value of <0.05 was considered statistically significant. Results: Nuclear and/or cytoplasmic p16INK4A immunoreactivity was absent in normal cervical tissue, while immunopositivity was 25% in CIN 1, 50% in CIN 2, 75% in CIN 3, 75% and 100% in squamous cell carcinoma and adenocarcinoma respectively. All normal cervical samples had low scores (0 to 2 points), while number of cases with high scores was proportional to the degree of cervical dysplasia or invasive carcinoma. p16INK4A immunostaining with higher expression (score>5) was observed in 75% cases of carcinoma cervix. Conclusion: IHC expression of p16INK4A is related to degree of histological dysplasia and malignancy, suggesting its prognostic and predictive value in the management of cervical cancers.
Authors and Affiliations
vatsala kishore, Anuradha G. Patil
Keywords
Related Articles
- EP ID EP362931
- DOI 10.7860/JCDR/2017/29394.10644
- Views 58
- Downloads 0
Labour Epidural Analgesia: A Randomised Double Blind Comparative Study of 0.1% Levobupivacaine with Fentanyl vs. 0.1% Ropivacaine with Fentanyl
Introduction: Levobupivacaine and ropivacaine are suitable alternatives to bupivacaine for labour analgesia as they produce less motor blockade, decreased incidence of instrumental deliveries and less toxicity. Aim: To s...
Significance of Morphometric Evaluation of Pre-Malignant and Malignant Lesions of Uterine Cervix: A Cross-sectional Analytical Study
ABSTRACT Introduction: Morphometric analysis of various parameters of the cells is done to classify the lesion as benign or malignant. It is an objective assessment which reduces the variability due to subjective visual...
A Case Report of Largest Documented Multilocular Cystic Nephroma Removed by Thoracoabdominal Approach
Large renal tumours are not uncommon in developing countries. Enhancing renal tumours are considered to be malignant unless proved otherwise and radical surgical resection remains the mainstay of treatment of such tumour...
Eye Donation Awareness and Conversion Rate in Hospital Cornea Retrieval Programme in a Tertiary Hospital of Central India
Introduction: Corneal blindness accounts for 6–8 million blinds in the world. In India, it is estimated that there are approximately 6.8 million people who have vision less than 6/60 in at least one eye due to corneal di...
Recurrent Postprandial Hypoglycaemia in Insulin Autoimmune Syndrome
ABSTRACT Insulin autoimmune hypoglycaemia syndrome (IAS) is not a common entity. It is characterised by auto-antibodies to endogenous insulin in persons without previous exposure to exogenous insulin. IAS is relatively c...