Expression of TGF-β1 During Fracture Repair in Ovariectomized Rats with Therapy of Cissus Quadrangularis Extract
Journal Title: Journal of Physiology and Pharmacology Advances - Year 2015, Vol 5, Issue 11
Abstract
This study aims to determine the osteogenic potential of Cissus quadrangularis extract based on its ability to increase the expression of TGF beta-1 in the case of osteoporotic fractures. This study used fourty female rats weighing 200–250 g. The rats were acclimatized for ten days, before they were randomly assigned to the sham-operated (SX, n = 10) or ovariectomized (n = 30) groups. The treatment started to be given the next day after osteotomy for six weeks. Rats in P0(NOV) and P1(OV) group received 1.5 ml 0.5% NaCMC per-oral as placebo. P2 group was treated using Raloxifene 5,4 mg/kg of body weight and P3 group was treated using Cissus quadrangularis extract 750 mg/kg of body weight. Examination of expression of TGF- β1 in this study performed twice. The first examination was done two weeks after osteotomy and the second examination was done six weeks after osteotomy.Based on the statistical test of the TGFβ-1 expression at the 2nd week, the highest score belongs to the P0 group (NOV) that have significant difference (p<0.05) with other groups of treatments, while the lowest score belongs to group P1 (OV) that have significant difference (p<0.05) with other groups of treatments. There are no significant difference at the P2 and P3 groups (p>0.05).From the expression of TGFβ-1 at the 6th weeks, the same pattern occurs to the 2nd week, the highest expression belongs to the P0 groups (NOV), and the lowest belongs to the P1 (OV) that have significant different with the other groups. The comparison of TGFβ-1 expression between the 2nd and the 6th weeks at almost all of the groups shows no significant different (p>0.05). The concluded that the extract of Cissus quadrangularis has the potential osteogenic that is able to stimulate the proliferation and differentiation of osteoblasts from mesenchymal stem cells, but it also is able to increase the activity of osteoblasts in bone formation because it can increase the expression of Transforming Growth Factor β1 (TGF-β1).
Authors and Affiliations
B. S. Lukiswanto , M. Z. Arifin , I. S. Yudaniayanti
Keywords
Related Articles
- EP ID EP127967
- DOI -
- Views 82
- Downloads 0
Hepatoprotective Effect of Glycyrrhiza Glabra in Carbon Tetrachloride-Induced Model of Acute Liver Injury
Acute liver injury is a serious state of extensive damage of liver tissue caused by various reasons. Certain medicinal plants had been used to cure some liver diseases. The present study was done to evaluate the hepatopr...
Stimulatory Effects of the Extract of Arachis Hypogaea (Fabaceae) on the Proliferation of Osteoblastic Cells in Vitro
Extracts of Arachis hypogaea (Fabaceae) have been studied in order to search for their effects on the metabolism of osteoblastic cells. ROS 17/2.8 osteoblastic cells were cultured in plates in standard medium and plant e...
Effect of Exercise and Quercetin on Obesity Induced Metabolic and Renal Impairments in Albino Rats
Obesity has been described as a state of chronic oxidative stress and inflammatory reaction. This study was carried out to clarify the effect of exercise and antioxidant quercetin on oxidative stress and inflammatory rea...
Cardioprotective Effects of Potassium Channel Openers on Rat Atria and Isolated Hearts under Acute Hypoxia
Under hypoxia, sarcolemmal KATP (sarcKATP) and mitochondrial KATP (mitoKATP) channels exert cardioprotective effects via different mechanisms. In the present study, effects of potassium channel openers (KCOs) on electrop...
Effects of the Aqueous Fruit Extract of Solanum macrocarpum Linn. on Hematological Parameters of Chronic Triton-Induced Hyperlipidemic Rats
The dried and pulverized unripe fruit of Solanum macrocarpum (Solanaceae) was soxlet-extracted with distilled water. Studies were then undertaken on the effect of the aqueous extract on hematological parameters and the l...