Expression of TGF-β1 During Fracture Repair in Ovariectomized Rats with Therapy of Cissus Quadrangularis Extract
Journal Title: Journal of Physiology and Pharmacology Advances - Year 2015, Vol 5, Issue 11
Abstract
This study aims to determine the osteogenic potential of Cissus quadrangularis extract based on its ability to increase the expression of TGF beta-1 in the case of osteoporotic fractures. This study used fourty female rats weighing 200–250 g. The rats were acclimatized for ten days, before they were randomly assigned to the sham-operated (SX, n = 10) or ovariectomized (n = 30) groups. The treatment started to be given the next day after osteotomy for six weeks. Rats in P0(NOV) and P1(OV) group received 1.5 ml 0.5% NaCMC per-oral as placebo. P2 group was treated using Raloxifene 5,4 mg/kg of body weight and P3 group was treated using Cissus quadrangularis extract 750 mg/kg of body weight. Examination of expression of TGF- β1 in this study performed twice. The first examination was done two weeks after osteotomy and the second examination was done six weeks after osteotomy.Based on the statistical test of the TGFβ-1 expression at the 2nd week, the highest score belongs to the P0 group (NOV) that have significant difference (p<0.05) with other groups of treatments, while the lowest score belongs to group P1 (OV) that have significant difference (p<0.05) with other groups of treatments. There are no significant difference at the P2 and P3 groups (p>0.05).From the expression of TGFβ-1 at the 6th weeks, the same pattern occurs to the 2nd week, the highest expression belongs to the P0 groups (NOV), and the lowest belongs to the P1 (OV) that have significant different with the other groups. The comparison of TGFβ-1 expression between the 2nd and the 6th weeks at almost all of the groups shows no significant different (p>0.05). The concluded that the extract of Cissus quadrangularis has the potential osteogenic that is able to stimulate the proliferation and differentiation of osteoblasts from mesenchymal stem cells, but it also is able to increase the activity of osteoblasts in bone formation because it can increase the expression of Transforming Growth Factor β1 (TGF-β1).
Authors and Affiliations
B. S. Lukiswanto , M. Z. Arifin , I. S. Yudaniayanti
Keywords
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