Expression of the bovine neutrophil β-defensins4 (BNBD4) and 10 (BNBD10), and β-defensin 1(DEFB1) in the bovine mammary gland with chronic mastitis by Staphylococcus aureus
Journal Title: Scientific Journal of Veterinary Advances - Year 2016, Vol 5, Issue 1
Abstract
Bovine mastitis is the most costly infectious disease of dairy cattle worldwide and ischaracterized by inflammation of the mammary gland. The etiology of the pathogens influences the severity of the inflammation and the immune response, and specifically some elements of the innate immunity, like the antimicrobial peptides, are known to display some pathogen specificity. The antimicrobial peptides play an important role in the innate immune defense mechanisms of the mammary gland against mastitis causative microorganisms. In the present study the expression of three β-defensins was estimated across 5 localizations of the mammary gland infected with Sthapylococcus aureus, by quantitative real-time PCR. Copies of mRNA for BNBD10 and DEFB1were detected in Lymph node, parenchyma, streak canal, cisterna, andRosette of Furstenberg, with the highest level observed in lymph node and the lowest in Rosette of Furstenberg, and the level of expression was higher for BNBD10. The level of mRNA expression for BNBD4 was very low in all localizations, with respect to BNBD10 and DEFB1. Therefore, BNBD10 and DEFB1 could play a key role in the mammary gland with mastitis by S. aureus.
Authors and Affiliations
A. R. Tamayo-Sosa*| Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, Mexicali, Baja California, México. Km 3.5 Carretera a San Felipe, Fraccionamiento Campestre S/N, México., V. M. Villar-Pérez| Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, Mexicali, Baja California, México. Km 3.5 Carretera a San Felipe, Fraccionamiento Campestre S/N, México., L. C. Pujol- Manríquez| Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, Mexicali, Baja California, México. Km 3.5 Carretera a San Felipe, Fraccionamiento Campestre S/N, México., L. Tinoco-Gracia| Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, Mexicali, Baja California, México. Km 3.5 Carretera a San Felipe, Fraccionamiento Campestre S/N, México., J. A. Olivas-Valdez| Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, Mexicali, Baja California, México. Km 3.5 Carretera a San Felipe, Fraccionamiento Campestre S/N, México., T. Melgarejo-García| Department of Human Nutrition, Kansas State University, Manhattan, KS, USA.
Keywords
Related Articles
- EP ID EP932
- DOI 10.14196/sjva.v5i1.2083
- Views 452
- Downloads 27
Kola nut (cola acuminata) extract as a substitute to histological tissue stain eosin
The application of natural dyes for staining of various biological tissues from an alternative source will decrease the expense for purchasing the synthetic dye and reduce their effects on human and environment. Therefor...
Expression of the bovine neutrophil β-defensins4 (BNBD4) and 10 (BNBD10), and β-defensin 1(DEFB1) in the bovine mammary gland with chronic mastitis by Staphylococcus aureus
Bovine mastitis is the most costly infectious disease of dairy cattle worldwide and ischaracterized by inflammation of the mammary gland. The etiology of the pathogens influences the severity of the inflammation and the...
Prevalence of setariosis in small and large ruminant in Miyaneh city, Northweast of Iran
Nematode Setaria belongs to Spirurida order and Setariidae family. The adult worms live in abdominal cavity freely (without clinical signs) and their sheathed microfiller exist in blood of their definitive hosts. Microfi...
Determination of larvae development stages in rainbow trout (oncorhynchusmykiss walbaum, 1792) based upon morphologic observations, pigment distribution patterns, and fin development patterns
Our knowledge aboutfish biology couldn't be completed without our awareness of bio- history oflarvae. The study of fish's bio- history can provide information about fishlarvae actions and reactions in its first life stag...
Lactational cadmium exposure induced alterations in the hematological indices and oxidative status in brain, liver and testes of rat pups
This study was carried out to investigate the effect of lactational exposure to cadmium (Cd) on suckling male rat pups. Hematological parameters, antioxidant status, some biochemical and pathophysiological indices in br...