Kola nut (cola acuminata) extract as a substitute to histological tissue stain eosin
Journal Title: Scientific Journal of Veterinary Advances - Year 2012, Vol 1, Issue 2
Abstract
The application of natural dyes for staining of various biological tissues from an alternative source will decrease the expense for purchasing the synthetic dye and reduce their effects on human and environment. Therefore, the objective of this study was to investigate the extraction of natural dye from Cola nut (Cola acuminata) using various solvents and its staining property on the rat tissues. The cola nut was pulvized using pestle and mortar, 5gram was used to make 5% of aqueous extract. The rat tissues were processed for paraffin embedding technique and sectioned at 5 μm thicknesses. The sections were stained with haematoxylin and the extracts as secondary stain. The results showed that the natural extract from Cola acuminata stained the cytoplasm of various tissues with yellowish-brown colouration. This finding suggests that Cola acuminata can be used as an alternative dye for histological staining.
Authors and Affiliations
S. A. Shehu| Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto, M. L. Sonfada| Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto, A. Danmaigoro| Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto, A. A. Umar| Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto, S. A. Hena| Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto, I. M. Wiam| Department of Veterinary Anatomy, University of Maiduguri, Maiduguri
Keywords
Related Articles
- EP ID EP860
- DOI -
- Views 804
- Downloads 46
Risk practices and awareness of leptospirosis in an abattoir in northwestern Nigeria
The study was designed to assess the presence of risk practices and level of awareness of leptospirosis among abattoir workers. The study was conducted in an abattoir located in Northwestern Nigeria. Pretested questionna...
Prevalence of lead (pb) residues in kidney and liver of slaughtered pigs at Sabo-Wakama market of Akun development area of Nasarawa state, Nigeria
The prevalence of Lead (Pb) in liver and kidney samples of slaughtered pigs at Sabo Wakama Market of Akun-Development Area of Nasarawa State was determined using Atomic Absorption Spectrophotometer. A total of thirty num...
Growth performance and linear body measurements of Washera, Farta and their crossbreed sheep under farmers management system in Western Highland of Amhara Region
The study was conducted in Farta and Lay Gaint districts ofthe Amhara Region. Data collected from 2007 to 2010 was used to evaluate growthperformance and to characterize phenotypically using linear body measurementsof Wa...
Effect of different energy sources on feed consumption, weight gain and feed conversion ratio in broilers
The 400 straight run, day old Vencobb broilers chicks were randomly divided into four treatment groups of 100 birds each viz., control, T1, T2 and T3. Each group was further divided into two replications of 50 birds each...
Serodiagnosis and molecular survey on letospiral abortions from sheep farms in Tabriz and surrounding areas
Leptospirosis is presumed to be the most widespread zoonosis in the world. The purpose of this study was to determine of seroprevalence of leptospirosis among the aborted ewes and in the same time, detection of bacterial...