Extended Spectrum Beta – Lactamase (ESBL) Mediated Resistance to Antibiotics Among Klebsiella Pneumoniae in Enugu Metropolis
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2009, Vol 2, Issue 2
Abstract
Aim. The objectives of this study were to determine the prevalence and antibiotic susceptibility patterns of extended – spectrum b - Lactamase (ESBL) producing Klebsiella pneumoniae isolated from three major diagnostic laboratories in Enugu State metropolis, Nigeria. Material and Methods. Clinical isolates of Klebsiella pneumoniae were obtained from urine, sputum and blood samples of patients attended diagnostic laboratories in Enugu metropolis. The isolates were subjected to susceptibility testing using Kirby and Bauer method of determining antimicrobial susceptibility and ESBL production was phenotypically determined using double disc synergy test. Results. A total of 300 clinical isolates of Klebsiella pneumoniae were isolated from three major diagnostic laboratories in Enugu namely Ambulin (100 urine samples), Mendex (100 blood sample) and Edisson (100 sputum samples) within a four month period (January – April 2009). ESBL production was determined among 300 isolates of Klebsiella pneumoniae and 186 (62%) Klebsiella pneumoniae express ESBL. ESBL producing Klebsiella pneumoniae were most frequently isolated from blood 76 (40.0%) followed by urine 66 (30.5%) and sputum 44 (23.6%). Resistance patterns ofKlebsiella pneumoniaerevealed that 64% were resistance to sparfloxacin, 92.6% to gentamicin, 90.9% to fusidic acid, 82.3% to erythromycin, 79% to trimethoprim, 96.3% to sulphamethoxazole, 88.5% to tetracycline 31.2% to nitrofurantoin, 31.2% to ciprofloxacin, 69% to ceftazidime, 74% to cefotaxime, 79.6% to ceftriaxone and 0% to imipenem. Conclusion. ESBL producing Klebsiella pneumoniae were present in these diagnostic laboratories and were resistant to different classes of antibiotics resulting in limited treatment options. Therefore we suggest that it will be important to perform screening and confirmatory tests for ESBL detection to any organisms resistant to any of the second and third generation cephalosporins in a routine diagnostic laboratory work.
Authors and Affiliations
Iroha Ifeanyichukwu Romanus| Department of Applied Microbiology, Ebonyi State University, P.M.B 053, Abakaliki Ebonyi State, Nigeria, Oji Anthonia Egwu| Department of Applied Microbiology, Ebonyi State University, P.M.B 053, Abakaliki Ebonyi State, Nigeria, Afiukwa Tifani Ngozi| Department of Applied Microbiology, Ebonyi State University, P.M.B 053, Abakaliki Ebonyi State, Nigeria, Nwuzo Agabus Chidiebube| Department of Applied Microbiology, Ebonyi State University, P.M.B 053, Abakaliki Ebonyi State, Nigeria, Ejikeugwu Peter Chika| Department of Applied Microbiology, Ebonyi State University, P.M.B 053, Abakaliki Ebonyi State, Nigeria
Keywords
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- EP ID EP8695
- DOI 10.3889/MJMS.1857-5773.2009.0052
- Views 334
- Downloads 21
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