Extended Spectrum Beta Lactamase and AMP C Beta Lactamase Production among Gram Negative Isolates Obtained from Burn Infections
Journal Title: International Journal of Contemporary Medical Research - Year 2017, Vol 4, Issue 6
Abstract
Introduction: Extended spectrum β -lactamases (ESBLs) and AmpC beta lactamses continue to be a major problem in health care settings. Due to the scarcity of information regarding the antibiotic susceptibility patterns particularly from burn infections, the current study was carried out to assist the clinicians to prescribe appropriate antibiotics against gram-negative clinical isolates. Material and methods: In the current study, a total of 210 samples were collected from burn patients. A total of 134 bacterial isolates were obtained during study period of one year, out of 134 bacterial isolates 114 gram negative isolates were screened for ESBL and MBL production. In the AST testing, the isolates that exhibited reduced zone of inhibition to one or more of the antibiotics such as cefotaxime (≤27mm), ceftriaxone (≤25mm), ceftazidime (≤22 mm), cefpodoxime (≤17mm) and aztreonam (≤27mm) were considered as potential ESBL producers and the ESBL production was confirmed using phenotypic screening test (double disk synergy test) and phenotypic confirmatory test (combined disk test). However, isolates showing resistance or decreased sensitivity to cefoxitin, cefotaxime, ceftriaxone, ceftazidime, cefpodoxime or aztreonam and sensitive to cefepime were considered as a screen positive AmpC producer and subjected to AmpC disk tests. Results: The current study showed that 33.3% and 14% of ESBL and AmpC producers were detected respectively in our hospital. Conclusion: Routine screening for ESBL and AmpC production should be done as theses are simple, easy to perform and interpret requiring less expertise.
Authors and Affiliations
Narinder Kaur, Shubhdeep Kaur
Keywords
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