Extended-Spectrum Beta-Lactamase-Producing E. Coli and Klebsiella Pneumoniae in Children at University Pediatric Clinic in Skopje
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2009, Vol 2, Issue 1
Abstract
Aim. The objectives of this study were to determine the prevalence and antibiotic susceptibility patterns of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. Material and methods. E. coli and K. pneumoniae were obtained from all clinical samples of hospitalized children. Results. During one year period, 212 strains of E. coli and 103 strains of K. pneumoniae were isolated. Of these, the ESBL production was observed in 26 (11.8%) isolates of E. coli and 25 (24.3%) isolates of K. pneumoniae. ESBLproducing E. coli isolates were commonly recovered from the respiratory tract (21.4%) and urine (7.2%). ESBL-positive K. pneumoniae was commonly recovered from urine (38.5%) and respiratory tract (18.7%). ESBL-positive E. coli isolates were more susceptible to AMC (76%) and SXT (50%), than were the isolates of ESBLpositive K. pneumoniae (40% and 32%). Considering aminoglycosides, 92% of ESBL-positive E. coli and 60% of ESBL-positive K. pneumoniae were susceptible to amikacin vs. 23% and 40% of ESBL-positive E. coli and K. pneumoniae strains to gentamicin. ESBL-positive K. pneumoniae strains were more susceptible to ciprofloxacin (84%) than ESBL-positive strains of E. coli (38%). Cefepime shows the best in vitro activity of tested cephalosporins (58% for E. coli and 72% for K. pneumoniae). All isolates were susceptible to imipenem. Conclusion. ESBL-producing E. coli and K. pneumoniae are present in our hospital environment. It’s necessary and useful to perform screening and confirmatory tests for phenotypic detection of those organisms in a routine work. Most of ESBL-producers are resistant to many classes of antibiotic, resulting in limited treatment options.
Authors and Affiliations
Ana Kaftandzhieva| Institute of Microbiology and Parasitology, Medical Faculty, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia, Vesna Kotevska| Institute of Microbiology and Parasitology, Medical Faculty, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia, Gordana Jankoska| Institute of Microbiology and Parasitology, Medical Faculty, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia, Biljana Kjurcik-Trajkovska| Institute of Microbiology and Parasitology, Medical Faculty, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia, Zhaklina Cekovska| Institute of Microbiology and Parasitology, Medical Faculty, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia, Milena Petrovska| Institute of Microbiology and Parasitology, Medical Faculty, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia
Prevalence and Clinical Manifestation of Glucose-6- Phosphate Dehydrogenase Deficiency in Newborns with Hyperbilirubinemia in Mashhad, Iran
Background. Hyperbilirubinemia is a relatively common disorder among infants and one of the most common reasons is glucose- 6- phosphate dehydrogenase (G6PD) deficiency. Aim. The aim of this study was to determine prev...
Macedonian International Medical Publications Indexed in PubMed in 2012
We present abstracts of published papers in international journals deposited in PubMed. Search details were: ((Macedonia[Affiliation] NOT Greece[Affiliation]) NOT "Prilozi"[Journal]) AND ("2012/01/01"[PDAT] : "2012/12/...
Cutaneous Manifestations of Brucellosis
Aim. To present and compare 10-year clinical experience of human brucellosis in Republic of Macedonia with data presented in the literature about the frequency of cutaneous manifestations of brucellosis. To estimate th...
Prognostic Value of the Expression of p53 in Patients with Non-small Cell Lung Cancer
Background. The p53 gene is frequently mutated in non-small-cell lung cancer (NSCLC). However, the effect of p53 gene mutations on patient prognosis remains unclear. Aim. The aim of this study is to determine the assoc...
Ocimum Gratissimum Linn Causes Dose Dependent Hepatotoxicity in Streptozotocin-Induced Diabetic Wistar Rats
Aim: Hepatoprotective effects of Ethanolic extract Ocimum gratissimum leaves (O. gratissimum) in streptozotocin-induced diabetic rats was studied. Material and Methods: Thirty-six adult rats were assigned into six grou...