Abstract

Objective: In this investigation, we demonstrated a simple one step method for metal mediated bacterial nanoparticles. Experimental approach: The aqueous solution of silver ions was treated with extracellular filtrate of E. coli and Proteus mirabilis biomass for the synthesis of nanoparticles. The gradual colour change from pale yellow to deep brown is a clear indication for the nanoparticle synthesis. The periodic checking of increase in pH range (alkaline pH) and optical density also determined showed the complete formulation of the structural data of nanoparticles. In vitro antibacterial and pathogenicity inhibition were determined. Findings and Discussion: This color change showed effective in E. coli. Among the bacteria tested, E. coli seen to be effective in the production of silver nanoparticles by reaching a pH of 9.6 after 28hours of incubation. The antimicrobial activity by well cutting method showed maximum inhibition of bacterial pathogen including E. coli, Staphylococcus aureus, Klebsiella pneumoniae and Proteus vulgaris, P. mirabilis, Acinetobacter baumanii species, whereas less inhibition was observed in Pseudomonas aeruginosa, Salmonella typhi and Vibrio cholerae species. In the case of pathogenicity inhibition study, the interaction of the produced nanoparticles with preconfirmed catalase and coagulase producing Staphylococcus aureus showed certain range of inhibition at the concentration of 1.5%. Capsulation of Klebsiella pneumoniae and swarming nature of Proteus also controlled. Conclusion: This showed that the synthesized nanoparticles may have potential action to lose the virulence of the test bacterial pathogen. Further investigation required to analyze whether the nanoparticles have the efficiency to reduce the virulence completely by in vivo methods. This analysis may provide the roadmap for the vaccine synthesis for various infections.

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