Extracellular Lipolytic Enzyme Production by a Novel Extremely Halophilic Bacterium
Journal Title: Universal Journal of Environmental Research and Technology - Year 2012, Vol 2, Issue 3
Abstract
Halophilic bacteria live in extreme salt conditions and can produce enzymes which are highly stable. A study was conducted to isolate lipolytic enzyme producing halophilic bacteria from salted fish. As lipolytic enzymes from halophiles can tolerate extreme salt conditions, it is highly stable and can tolerate harsh industrial conditions and thus has got potential biotechnological applications. In the present work 17 halophilic isolates were obtained from salted fish samples on Seghals Gibbons Complex (SGC) medium. The lipolytic activity of the strains was tested by Tween hydrolysis on SGC medium containing 1% Tween 80 or Tween 40. A strain SH1 which showed the highest relative enzyme activity was identified by 16 S rDNA sequencing as a novel strain belonging to the class Gamma proteobacteria. The strain was found to grow in a salt range, 2 to 5 M and thus confirmed as an extreme halophile. It showed lipolytic activity at salt concentration in the range 2 to 4 M and pH range 6 to 11. Thus it forms a promising strain for the production of halophilic lipolytic enzymes and thus can have industrial applications.
Authors and Affiliations
Anisha C, Jyothis Mathew and Radhakrishnan E K
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