EXTRACELLULAR POLYMERIC SUBSTANCE (EPS) FROM KOCURIA SP. BRI 36: A KEY COMPONENT IN HEAVY METAL RESISTANCE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 5
Abstract
Objective: Evaluation of Extracellular Polymeric Substance (EPS) induced heavy metal tolerance in Kocuria sp. BRI 36.Methods: Initially, the effect of different concentrations of glucose (1-10 %) on EPS production by BRI 36 was examined. At optimum glucose concentrations, EPS levels were measured by varying heavy metal concentrations (10-50 ppm) of Pb2+, Cd 2+and Cr3+. Maximum tolerable concentration (MTC) and survival percentage of BRI 36 were determined under conditions that support EPS synthesis. Comparative analysis of extracted crude EPS was performed by Fourier Transform Infrared Spectroscopy (FTIR) to establish functional groups involved in the metal interaction. Results: Kocuriasp. BRI 36 produced maximum EPS (1g/l) at 5% glucose. Increase in EPS production up to 89% (considering 1g/l as 100%) with an increase in concentrations of heavy metals up to 40 ppm. MTC levels of BRI 36 for heavy metals increased up to 700 ppm when it was cultivated in presence of 5% glucose indicating a major role of surface polymer in metal adsorption. The function of EPS as a protective cover was also evident from an increase in survival percentage of BRI 36 up to 39.4 at MTC. Comparative analysis of extracted crude EPS by FTIR revealed the involvement of O-H, C=O, and C-O-C groups in metal adsorptionConclusion: Antarctic oceanic isolate Kocuria sp. BRI 36 has an ability to produced EPS under stress conditions of heavy metals. Simultaneously, its MTC values increased due to increase in EPS levels. These observations suggest the possibility to develop gentle, environmentally safe and cost-effective method for heavy metal removal.
Authors and Affiliations
Anuradha Mulik, Rama Bhadekar
Keywords
Related Articles
- EP ID EP567497
- DOI 10.22159/ijpps.2018v10i5.23535
- Views 65
- Downloads 0
PHYTOCHEMICAL EVALUATION AND PHARMACOLOGICAL ACTIVITY OF SYZYGIUM AROMATICUM: A COMPREHENSIVE REVIEW
Medicinal plants are generating an ever-increasing amount of interest due to the effectiveness, low cost and minimal side-effects associated with drugs derived from them. Clove (Syzygium aromaticum (L.) (Family Myrtaceae...
FLOATING-BIOADHESIVE MATRIX TABLETS OF HYDRALAZINE HCL MADE OF CASHEW GUM AND HPMC K4M
Objective: The objective of this paper was to prepare and evaluate floating-bioadhesive cashew gum-hydroxypropyl methylcellulose (HPMC K4M) matrix tablets for the gastro-retentive release of hydralazine HCl.Methods: The...
DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DULOXETINE
Objective: The aim of the present work was to develop and validate a simple UV spectroscopic method for the determination of duloxetine, which is a thiophene derivative and a selective neurotransmitter reuptake...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CURCUMIN AND CYCLOSPORINE BY RP-HPLC
Objective: The present work was undertaken with an aim to develop and validate a rapid reverse-phase high-performance liquid chromatography (RP-HPLC) method for the estimation of curcumin and cyclosporine in the capsule...
NEUROTOX: A UNIQUE DATABASE FOR ANIMAL NEUROTOXINS
Objective: A wide range of toxic effects against other species are affected through potential protein molecules called neurotoxins. Among various kinds of toxins, neurotoxins play a critical role in life of other organis...