Extraction and comparison of quality and purity of DNA of medicinal plants by CTAB and kit method
Journal Title: Journal of Contemporary Pharmacy - Year 2022, Vol 6, Issue 1
Abstract
Background: Today is a world of bioengineering. Man is playing with DNA. Extraction and isolation of DNA has become as a key part for the researches of modern times. One needs high quality and increased quantity of DNA for performing several genetic techniques. There are several methods through which DNA can be extracted but the quality of the DNA cannot be compromised. Material methods: In the current research work extraction of DNA from four medicinal important plants Cassia fistula, Saccharum officinarum, Albizzia lebbeck and Cymbopogan citratus was carried out by two methods. One is a manual method that is used for the extraction of DNA from plant source; The CTAB method, and the other is extraction with the help of commercially available kit. Results: Results of the work showed that the high quantity of the DNA was obtained by CTAB method while DNA extracted with the kit was of better quality and purity then CTAB method. Conclusion: In techniques where high quality DNA is required like genetic engineering, micro arrays, extraction of DNA through kit method should be preferred, while in cases where quantity of DNA maters over its quality CTAB method is better to be used and economic.
Authors and Affiliations
Raheela Jabeen, Ume Habiba, Sana Khalid, Jafir Hussain Shirazi
Keywords
Related Articles
- EP ID EP713756
- DOI https://doi.org/10.56770/jcp2022613
- Views 63
- Downloads 0
Formulation and evaluation of 5-fluorouracil controlled release chronotherapeutic drug delivery system (CTDDS) for colorectal cancer
Objective: As most of the drugs disintegrate and dissolve in stomach before reaching the target site and to substitute intravenous (IV) route based chrono modulated chemotherapy, oral colon target drug delivery system wa...
Preparation techniques, functionalization, and cytotoxicity of magnetic nanoparticles: A review
In the relatively new but fast-evolving field of nanomedicine and nanotransport systems, different nanoscale molecules are used to deliver therapeutic agents to precisely targeted sites or potentially function as diagnos...
Tuberculosis in geriatric patients
Tuberculosis is developing as a noteworthy medical problem in geriatrics. The indications are frequently nonparticular and prompt postponed finding. Propelled infection state exhibit at the time of determination and regu...
Formulation and evaluation of fast dissolving films embedded with nanoparticles of mirtazapine hydrochloride
Objectives: The goal of this research was to produce fast dissolving film embedded with nanoparticles of mirtazapine hydrochloride. Method: Semisynthetic polymer (HPMC) is used to prepare orodispersible films which is pr...
Extraction and comparison of quality and purity of DNA of medicinal plants by CTAB and kit method
Background: Today is a world of bioengineering. Man is playing with DNA. Extraction and isolation of DNA has become as a key part for the researches of modern times. One needs high quality and increased quantity of DNA f...