EXTRACTION OF FLAVONOIDS FROM BUCHANANIA LANZAN SPRENG. SEEDS BY SUPERCRITICAL FLUID EXTRACTION AND DETERMINATION OF THEIR ANTIOXIDANT ACTIVITY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 1
Abstract
Objective: The purpose of the study is to optimize supercritical fluid extraction (SFE) conditions for the flavonoids from the seeds of Buchanania lanzan Spreng. followed by quantitative determination of the antioxidant ability of the supercritical fluid (SCF) extract.Methods: The conditions optimized for supercritical fluid extraction (SFE) were temperature, pressure, carbon dioxide (CO2) flow rate and co-solvent percentage. High-performance liquid chromatography (HPLC) method was used to determine the flavonoid content at each condition for optimization. Chromatographic conditions were mobile phase–Methanol: Acetonitrile: Orthophosphoric acid: Acetic acid: Water (200:100:10:10:200 v/v), flow rate–1 ml/min, column–Kromasil C18, 250 x 4.6 mm and detection was done in PDA range. Quantitative estimation of polyphenols was done spectrophotometrically. In vitro antioxidant ability of the extract was checked using free radical scavenging activity by 1, 1–Diphenyl–2–picrylhydrazyl (DPPH) assay and ferric reducing power (FRAP) assay.Results: The optimum supercritical fluid extraction conditions were temperature 35 °C, pressure 19.61 MPa, carbon dioxide flow rate 3 ml/min and co-solvent 5.66 %. The extraction yield obtained was 20.50±0.47 %. The polyphenolic content was 52.14±0.7 mg Gallic acid equivalents (GAE)/g extract. EC50 value for free radical scavenging activity was 124.58±1.6 µg/ml, and ferric reducing capacity was 456.06±5.61 µg/ml.Conclusion: Supercritical fluid extraction (SFE) technique could be used as an alternative technique for obtaining the maximum yield of flavonoids from the seeds of Buchanania lanzan. The results showed that the supercritical fluid (SCF) extracts exhibited good antioxidant activity which could be due to the presence of polyphenols and flavonoidsÂ
Authors and Affiliations
Roshani Y Vyavaharkar, S. S. Mangaonkar
Keywords
Related Articles
- EP ID EP577968
- DOI -
- Views 53
- Downloads 0
SCREENING OF PHYTO-CHEMICALS, TLC PROFILING, TOTAL FLAVONOID AND PHENOLICS CONTENT, ANTI-OXIDANT ACTIVITY AND ANTI-MICROBIAL ACTIVITY OF FICUS BENGHALENSIS LINN AND FICUS RELIGIOSA LINN LATEX
Objective: Screening for the presence of phyto-chemicals present in the plant extracts by qualitative assay along with Thin Layer Chromatography (TLC) investigation followed by determining the total flavonoids and phenol...
APPLICATION OF NANOSILVER FOR PREVENTION OF RECURRENT DENTAL CARIES IN PATIENTS SUFFERING FROM XEROSTOMIA.
Objective: Prevention of recurrent caries due to decreased oral clearance and an abnormal microbial milieu in patients suffering from xerostomia poses a significant challenge in restorative dentistry. The present study w...
COMPARATIVE PHYTOCHEMICAL EVALUATION OF ETHNOBOTANICALLY IMPORTANT MEDICINAL PARASITIC HERB: ALECTRA CHITRAKUTENSIS
Objective: Alectra chitrakutensis (M. A. Rau) R. Prasad & R. D. Dixit is a critically endangered and ethnobotanically very important plant. Official drug i.e. dried rhizome of the plant has been reported to be used f...
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF ASPIRIN, ROSUVASTATIN, CLOPIDOGREL IN BULK AND PHARMACEUTICAL DOSAGE FORM
Objective: To develop a novel, accurate, precise and linear reverse phase high-performance liquid chromatography (RP-HPLC) and Stability Indicating Assay Method (SIAMs) for simultaneous, qualitative and quantitative esti...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR QUANTIFICATION OF FLUNISOLIDE IN NASAL SPRAY FORMULATION BY RP-HPLC
Objective: The objective of the present research work was to develop and validate reversed-phase high-performance liquid chromatography (RP-HPLC) method for quantification of flunisolide in nasal spray formulations.Metho...