EXTRACTION OF INVERTASE FROM SACCHAROMYCES CEREVISIAE: EFFECT OF RHEOLOGICAL PARAMETERS
Journal Title: World Journal of Pharmaceutical Research - Year 2019, Vol 8, Issue 5
Abstract
Baker’s yeast cells were suspended in 50 mL of 0.154M NaHCO3 and left under agitation of 200cpm in rotary and reciprocal type-shakers at 45oC for 24h. The yields of invertase obtained through reciprocal and rotary shakers were 33.2% and 46.0%, respectively. The turbulent regimen observed in the reciprocal shaker denatured the invertase more intensely than the laminar regimen occurred in the rotary shaker. A correlation was observed between the scale factors (Reynolds and Froude numbers, symbolized as NRe and NFr, respectively) and the final invertase activity attained. Thereby, NRe = 564 and NFr =1.20 led to obtain an extract with high total invertase activity (22U).
Authors and Affiliations
Prof. Michele Vitolo
Keywords
Related Articles
- EP ID EP672195
- DOI -
- Views 126
- Downloads 0
FOLKLORE USES OF ACALYPHA INDICA IN AYURVEDA SYSTEM
Ancient days peoples are purely depends on herbal drugs. why because at that time allopathic drugs are not available for peoples. now a days both ayurveda and allopathic drugs are used some chronic diseases are completel...
VIRTUAL REALITY- A REVIEW
Sight and sound play a crucial role in our life. They serve as a tool to lead a better living. Without these, enjoying the beauty of life is impossible. It is a boon from Almighty to everyone. The values of these are ver...
PARTIALLY TREATED BACTERIAL MENINGITIS; THE DILEMMA CONTINUES
Objective: This study aimed to determine the effect of pretreatment with antibiotics before lumbar puncture on cerebrospinal fluid profile(CSF) compared with the CSF results’ of patients who were not pretreated with anti...
PREGNANCY OUTCOMES IN WOMEN WITH STRICTLY CONTROLLED TYPE 1 DIABETES MELLITUS
Prenatal care, glucose regulation, and regular antenatal visits are the mainstays of the management of diabetic pregnant patients. We offer and inform the diabetic patients about these management protocols before plannin...
DEVELOPMENT OF A NEW CHROMATOGRAPHIC METHOD FOR ESTIMATION OF DOLASETRON IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC
Background: A simple, accurate and precise HPLC method for simultaneous determination of Dolasetron in pure and tablet dosage form has been developed. Aim: Development Of A New Chromatographic Method For Estimation Of Do...