Factors affecting genetic transformation via Agrobacterium tumefaciens in Phaseolus vulgaris L.
Journal Title: Biotecnología Vegetal - Year 2013, Vol 13, Issue 2
Abstract
Phaseolus genus, as well as other legumes, has proved recalcitrant plant regeneration in vitro and m ai n l y t o genetic transformation via Agrobacterium tumefaciens. Genetic transformation in this species is limited because of their genotype dependent, as well as being a process of low efficiency and poor reproducibility. There are evidences that several factors influence DNA integration and stable transformation of plants. This review aims to analyze the influence of different factors on the efficiency of genetic transformation via Agrobacterium tumefaciens in Phaseolus such as: bacterial strain, plasmid, temperature, lighting conditions, coculture time, bacterial concentration and selection marker.
Authors and Affiliations
Amanda Martirena-Ramírez, Novisel Veitía
Keywords
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