Field evaluation of inactivated Corynebacterium ovis vaccine in sheep prepared from a local isolate
Journal Title: Journal of Veterinary Medical Research - Year 2016, Vol 23, Issue 1
Abstract
Corynebacterium pseudotuberculosis vaccine was prepared from a local field isolate. Vaccination of sheep with 50g PLD toxoid and 10 mg bacterin adjuvanted with Montanide oil improved the levels of immune responses of sheep. In many countries, inactivated C. pseudotuberculosis adjuvant vaccines have been used for prevention and control of caseous lymphadenitis in sheep. However, the efficacy was variable. The aim of the present study was directed to prepare and evaluate the potency of an inactivated C. pseudotuberculosis vaccine using Montanide ISA206. Sheep were vaccinated with 1st dose of 2ml containing 10 mg bacterin and 50g toxoid and Montanide ISA 206 oil adjuvant and boostered with the same dose 15 days Apart. Evaluation of post vaccinal cellular immune response with lymphocyte proliferation assay and humoral immune response using ELISA was carried out. Cell mediated immune response of vaccinated sheep reached its peak 0.445 by 1st week post the second vaccination. The level of humoral immune response showed optical density of 1.005 by 1st week post the second vaccination. Challenge test was done in all sheep four weeks after the second dose of vaccination. Three sheep from vaccinated and three sheep from non-vaccinated groups were slaughtered and necropsied 150 days post challenge. The results revealed 75% protection percentage against challenge while unvaccinated challenged sheep showed 9% protection. Statistical analysis indicated that the vaccine assessed a significant level of cellular and humoral immunity.
Authors and Affiliations
Nabila A. Ghazy, Mohamed G. Abdelwahab, Makhareta M. A. M. , Abdallah Y. A.
Further evaluation of locally prepared live attenuated bovine ephemeral fever vaccine in cattle
Bovine ephemeral fever (BEF) is an acute, arthropod-borne disease of cattle. The disease is characterized by sudden onset of fever, high morbidity and very low mortality. Recovery occurs within three days of the onset of...
Some studies on helminth parasites of buff backed heron (Ardeola ibis ibis) with special reference to its role in transmision of Clinostomum complanatum in Beni-Suef Governorate
A number of 50 Ardeola ibis ibis birds were found harboring six nematodes species; Tetrameres species, Microtteramere species, Synhimantus invaginatus, Synhimantus equispeculatus, Ascaridia species, Paracamallanus specie...
A case report of anatomical variation in the left kidney of a Pit Bull dog in Trinidad
The current study showed that the right and left kidneys of a Pit Bull dog were normal, the only difference, which appeared in the left kidney, is the bulging of the mid-lateral border with a marked depression in the cra...
EFFECT OF VIRGIN OLIVE OIL SUPPLEMENTATION ON LIPID PROFILE AND OXIDATIVE STATUS IN RATS
The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats supplemented with normal diet. thirty male Sprague Dawley rats, (90-110 g), were used in the pres...
Life cycle of Philophthalamus species for the first time in Egypt
The present investigation succeeded in following up the life cycle of Philophthalamus species cercaria emerged from Cleopatra bulimoides snail till production of adult worm Philophthalamus species. This adult worm was ex...