Fine mapping of genes based on molecular markers and high-throughput sequencing
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 10
Abstract
The rice spotted leaf mutant Spl34 is a naturally obtained mutant. The mutant Spl34 had been studied in the morphological, physiological and biochemical aspects of the mutant Spl34. The F2 gene localization population was constructed by hybridization of indica rice Francis and Spl34. Genetic analysis showed that the spotted leaf phenotype of Spl34 was regulated by a single dominant gene Spl34(t). The SSR/InDel marker was used to link the leafless leaf recessive plants, and Spl34(t) was finely mapped on chromosome 11 between 23.483 and 23.530 Mb. The length of the localization interval was 46.99 kb, and 8 candidate genes were screened. In addition, high-throughput sequencing technology was used to re-sequence the F2 (Francis/Spl34) spotted individual pool, the spotless individual pool and the parents, and the genomic SNP was used to correlate the spotted and non-spotted pools. Positioned on the 11th chromosome between 23.223-23.791 Mb, the interval size is about 568 kb; further Spl34 mutants were sequenced by single-molecule sequencing technology, and detected by de novo assembly and correlation analysis in two overlapping groups tig00001409 and tig00003011 to the close chain. The length of the new contig generated by Sanger sequencing of the sequences between the two contigs reached 233.95 kb, and the Spl34(t) localization interval was about 40 kb. In this study, the spotted leaf gene Spl34(t) to the 40 kb region of chromosome 11, which laid a good foundation for the further functional study of this gene.
Authors and Affiliations
Mao-hui WANG, Zhi-qiang CHEN
Keywords
Related Articles
- EP ID EP601589
- DOI 10.16768/j.issn.1004-874X.2018.10.001
- Views 70
- Downloads 0
Analysis on the reason and countermeasures of the downturn market of Guangxi banana in 2017
"This study combined national and Guangxi banana planting situation，and carried out in-depth investigation and research on the situation of banana planting，production and marketing and the incidence of fusarium wilt in...
Effects of planting density on yield traits and growth of indica rice NF11, aerect panicle type
"In order to investigate the differences of yield and yield traits of indica rice in erect panicle type under different planting densities, and provide a theoretical basis for the production of indica rice in erect panic...
Effects of harvest time on fruit quality and storability of ‘Red Sun’ kiwifruit
"The Red Sun kiwifruit is the most popular variety of kiwifruits，while this variety is also commonly found in markets，the fresh preservation technology limits its application in kiwifruit industrial development. Approp...
Effects of different culture medium on the growth of hybrid seeds of Phalaenopsis amabilis by aseptic seeding
"The effects of basic culture medium and organic additives on the germination，differentiation， seedling and rooting of different hybrid seeds of Phalaenopsis amabilis were investigated by vitro culture. The results dem...
Study on heavy mental thallium biosorption by common plants
"In this study，hydroponic experiments were conducted to investigate the uptake rates and accumulation of thallium（ Tl+ and Tl3+） in four types of plants and the effect of K+ on accumulation of Tl+ and Tl3+ in plant. T...