First isolation of biodegradable polycyclic aromatic hydrocarbons Mycobacterium porcinum and Mycobacterium celeriflavum from oil-polluted ecosystems
Journal Title: Environmental Health Engineering and Management Journal - Year 2022, Vol 9, Issue 2
Abstract
Background: Polycyclic aromatic hydrocarbons (PAHs) have detrimental effects on human, ecosystem, and biodiversity. Bioremediation is an option that has been used to remediate and reduce the risk of contaminants such as PAHs. Microorganisms are readily available to screen and can be rapidly identified to be used in many extreme environmental conditions. Mycobacteria have a great potential for the production of bioactive compound, which have degradation activity. Due to this issue, and also, as there is no study conducted on the biodiversity of biodegradable Mycobacterium in Markazi province, the present study aimed to assess the isolation and identification of biodegradable Mycobacterium species from diverse Markazi province ecosystems. Methods: Mycobacterium were screened from a total of 30 soil, water, and sludge samples from the oilpolluted ecosystems of Markazi province and characterized to the genus and species level by applying molecular and conventional microbiological assay including the PCR amplification and sequence analysis of 16SrRNA and hsp65 genes. The growth rate in the presence of PAHs, turbidometry, and high performance liquid chromatography (HPLC) were used to determine their bioremediation capability. Results: In total, 6 Mycobacterium isolates (20%) were screened from 30 samples, which belonged to two species of Mycobacterium consisting of M. porcinum (4 isolates) and M. celeriflavum (2 isolates). The strains of M. porcinum and M. celeriflavum could degrade 70% and 90% of 1 mg/L PAH solution in 7 days. Conclusion: According to the results, the M. porcinum and M. celeriflavum have a significant capability to biodegrade the PAHs. Therefore, more investigations are recommended for separation and applicational use of the mycobacterium species for bioremediation of PAHs.
Authors and Affiliations
Shiva Hosseini , Davood Azadi , Abdorrahim Absalan
Keywords
Related Articles
- EP ID EP705343
- DOI 10.34172/EHEM.2022.11
- Views 54
- Downloads 0
An environmentally friendly soil improvement technology for sand and dust storms control
Background: Dust storms occur when unchecked, strong, or turbulent winds combine with exposed loose and dried soil surfaces. Sand and dust storms have a significant impact on society, economy, and environment at local, r...
Removal of Acid Orange 7 dye from wastewater using combination of ultraviolet radiation, ultrasonic method, and MgO nanoparticles
Background: Industrial dyes are toxic and carcinogenic, therefore, they should be removed from wastewater. The aim of this study was to investigate the removal of acid orange 7 Dye from wastewater using ultraviolet (UV)...
A new treatment design for water contaminated with phenol
Background: Several important designs have been applied to remove toxic and hazardous organic substances like phenol and phenol compounds from wastewater, but there is a need to seek an alternative design to effectively...
Study of the effect of sodium alginate coating containing pomegranate peel extract on chemical, sensory and microbial quality of walnut kernel
Background: Due to the adverse effects of artificial preservatives on food and its harmful effects on human health, researchers have been considering replacing these materials with natural substances. In this study, the...
The effect of the use of disinfectants during COVID-19 pandemic on the bacterial contamination of dental unit waterlines
Background: The emergence and spread of SARS-CoV-2 has increased environmental disinfectant usage to reduce the transmission of this virus. Ethanol 70%-90% and 5% sodium hypochlorite have the highest consumption for di...