Flow Cytometric Immunophenotyping of Acute Leukaemia in Adult and Its Comparison with Cytomorphology
Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 5
Abstract
Background: Immunophenotyping has become an inseparable tool for precise characterization of acute leukemia (AL), which is the corner stone of therapeutic success in these cases. There is paucity of data regarding immunophenotypic profile of adult acute leukemia in Bangladeshi population. This study was carried out to see the immunophenotypic pattern of different types of AL in our population and also to compare between results of flow cytometric immunophenotyping and cytomorphologic assessment. Methods: A total of seventy (70) morphologically diagnosed adult AL cases (ageā„ 18 years) from Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka Medical College & Hospital (DMCH), Sir Salimullah Medical College & Mitford Hospital (SSMC & MH), and National Institute of Cancer Research & Hospital (NICRH) were analyzed by 4-color flow cytometry for immunophenotyping using a predefined panel of antibody. All the samples were reviewed for morphologic features by light microscope before flow cytometric assessment. Results: Flow cytometry could assign lineage to 64 cases out of 70. Among the 64 cases 31 (48.4%) cases were Acute Myeloblastic Leukaemia (AML), 31 (48.4%) cases were Acute Lymphoblastic Leukaemia (ALL), and 2 (3.12%) were mixed phenotype acute leukemia. CD13 and CD117 were the mostly expressed markers (96.8%) in the AML cases while CD19 was most frequent (100%) in B-ALL, and CD3 was mostly expressed (100%) in T-ALL. Aberrant antigen expression was found in 39.1% of the acute leukemia cases. Complete lineage agreement rate was higher in AML (89.7%) than that of ALL (80.6%). Complete Discordance was found in 9.4% cases between the results of flow cytometry and cytomorphology. Conclusion: Inclusion of flow cytometry in routine diagnostic workup of acute leukemia ensures proper characterization as well as management of these cases.
Authors and Affiliations
Asif Rashed, Shirin Tarafder, Humayun Sattar, Sadia Hossain
Keywords
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- EP ID EP536948
- DOI 10.21276/ijmrp.2018.4.5.037
- Views 67
- Downloads 0
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