Flow Cytometry In The Differential Diagnosıs Of Neutropenic Patients
Journal Title: Acta Medica Ruha - Year 2023, Vol 1, Issue 2
Abstract
Introduction:Although immuno-phenotyping with flow cytometry is mainly used in the diagnosis of hematological neoplasia, it also provides important contributions to revealing changes in the immune system. Flow cytometric studies are also frequently performed in neutropenic patients, as changes in neutrophil count may occur in relation to hematological neoplasms and immune system disorders.Objective:Within the scope of this study, we aimed to elucidate the expressions of lymphocyte, granulocyte, and monocyte subtypes and myelomonocytic antigens on monocytes and neutrophils in neutropenia caused by different reasons and compare them with the results of healthy volunteers.Method:A total of 24 patients and 13 age-and gender-matched healthy volunteers who applied to our institution have been enrolled in this study. The patient group consisted of individuals with isolated neutropenia. Examination findings, lymph node size, spleen size, and infection findings,and the laboratory parameters, CRP level, and pathological findings in the peripheral smear were recorded. Total blood obtained from the patients has been processed with flow cytometry.Results:Neutrophil/lymphocyte (N/L) MFI ratio was found to be higher in the patient group than in the control group (p=0.022).A positive correlation was found between leukocyte counts and the percentage of atypical monocyte expressing CD16 (p=0.033). A negative correlation was found between platelet counts and N/L mean fluorescence intensity ratio (p=0.411) and between the platelet count and the percentage of CD11b on neutrophils (p=0.026). Thepercentage of neutrophils determined on leukocytes and the percentage of natural killer cells (CD30/CD56+) was statistically significantly lower in the patient group (p=0.008, p=0.001 respectively).Conclusion:Regarding the results of this study one could say that clinically significant changes have been observed in lymphocyte and monocyte subtypes of patients with isolated neutropenia. Granulation was found in the ratio of N/L side scatter, mostly related to neutrophils.
Authors and Affiliations
Ferda Çelik
Keywords
Related Articles
- EP ID EP718171
- DOI 10.5281/zenodo.7908431
- Views 36
- Downloads 0
Subfertil Hastalarda Klomifen Sitrat ile Uygulanan Ovülasyon İndüksiyonunun Over Rezervini Ölçmeye Yarayan Testler Üzerindeki Etkisi
Giriş: Bu çalışmada Bazal FSH, Estradiol, ultrason ile ölçülen antral folikül sayımı (AFS) ve total over volümü (TOV) gibi over rezervi (OR) testlerinin Klomifen sitrat (KS) ile yapılan ovülasyon indüksiyonu sonrası deği...
Investigation of the Relationship of Wrist Range of Motion with 2D:4D Ratio in Healthy Individuals
Introduction: Range of motion (ROM) is widely used in the diagnosis of musculoskeletal disorders and in the evaluation of treatment prognosis. The 2D:4D ratio is the ratio of the second finger to the fourth finger. Ob...
Genişlemiş Spektrumlu Beta Laktamaz Üreten Escherichiacoli‘nin Neden Olduğu Üriner Sistem İnfeksiyonlarındaAynı Etkenin Dışkıda Taşıyıcılığının Saptanması
Giriş-Amaç: GSBL (+) E. coli‟nin neden olduğu ÜSİ‟de aynı etkenin dışkı kolonizasyonunun ve hem idrar hem de dışkı izolatlarından elde edilen fenotipik olarak benzer etkenlerin moleküler olarak birbirleriyle b...
Effect Of Home Visits by Nurses on Hemoglobin Levels Of 6-Month-Old Infants. A Nonrandomized Controlled Trial
Background: Iron-deficiency anemia has a high prevalence among infants across the world including our country and contributes substantially to the global burden of disease. Therefore, it is a priority to develop preventi...
Diyabetik ve Nondiyabetik Koroner Arter Hastalarında Kardiyopulmoner Bypass Öncesi ve Sonrası Troponin-I Düzeylerinin Karşılaştırılması - Comparison of Troponin-I Levels Before and After Cardiopulmonary Bypass in Diabetic and Nondiabetic Coronary Artery Patients
Giriş: Kardiyak troponin I (cTnI) miyokardiyal hasar için tanısal bir biyobelirteçtir.Amaç:Bu çalışmanın amacı diyabetik ve nondiyabetik koroner arter hastalarında KPB öncesi ve sonrası Troponin-I düzeylerini...