FLT3 Protein Expression and Mutation in B Cell Acute Lymphoblastic Leukemia (B-ALL)
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2015, Vol 17, Issue 2
Abstract
FLT3 is a receptor tyrosine kinase, which play important role in normal hematopoiesis activating mutations of FLT3 are commonly found in acute leukemia patients and reported to be associated with poor clinical outcome. This study aims to evaluate the incidence of FLT3 mutation and CD135 protein expression and their role in prognosis of B cell acute lymphoblastic leukemia (B-ALL). Detection of FLT3 internal tandem duplication (ITD) was carried out in total 32 patients diagnosed as B-ALL using reverse transcriptase - polymerase chain reaction (RT-PCR) method. The quantification of (CD135) FLT3 protein on leukemic blasts was done by flowcytometery in total 51 B-ALL patients. The incidence of FLT3 ITD mutation and CD 135 expression was to be found 19% and 51% respectively in 51 de novo B ALL patients. High incidence of FLT3 ITD mutation was noted in patients with low blast count, normal WBC count, low platelet count, low RBC count and low Haemoglobin level but showed no correlation with disease status. High incidence of CD135 over expression was noted in patients with high WBC count, low platelet count, low RBC count and low haemoglobin level and significant association was found with aberrant expression of CD13 and CD33. In relation to disease status, one patient showed disease relapse which had wild type FLT3 gene but CD135 over expression at diagnosis. CD135 over expression was found to be associated with poor prognostic markers and its presence at diagnosis in patient showing disease relapse confirms its role in disease aggressiveness of B-ALL. It might be suggested that CD135 protein is of more clinical relevance as compared to the FLT3 mutations
Authors and Affiliations
Khushbu Rajan, Shalvi Mehta, Hemangini Vora
Keywords
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