Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders
Journal Title: Open Veterinary Journal - Year 2013, Vol 3, Issue 1
Abstract
The aim of this study was to assess the effect on boar spermatozoa quality of in vitro storage in short and long-term extenders by fluorescent multiple staining (FMS) and computer assisted semen analyzer (CASA). Fresh ejaculates from three healthy, sexually mature boars were diluted with equal volumes of six short-term or three long-term commercial extenders and stored at 19°C for 6 days (short-term) or 12 days (long-term). The integrity of spermatozoa membranes was analyzed by FMS using propidium iodide, 5,5’,6,6’-tetrachloro-1,1’,3,3’ tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) and fluorescein isothiocyanate-conjugated peanut agglutinin (PNA). The results obtained from this staining were compared with spermatozoa motility assessed by CASA. Our study showed that the number of viable spermatozoa with non-reacted acrosomes and intact mitochondria was positively correlated with the rate of motile spermatozoa (r2>0.9) irrespective of the extender used. In all extenders the number of motile spermatozoa significantly decreased as preservation period increased (P<0.05). FMS test is a potent indicator of sperm motility because it analyses mitochondrial integrity independently from observable alterations in motility. The best performing extenders were BTS for short-term storage and TRI-x-Cell for long-term storage.
Authors and Affiliations
A. Lange-Consiglio| Università degli Studi di Milano, Faculty of Veterinary Medicine, Department of Health, Animal Science and Food Safety, Large Animal Hospital, Reproduction Unit, 6 Via dell'Università, I-26900 Lodi, Italy, A. Meucci and F. Cremonesi| Università degli Studi di Milano, Faculty of Veterinary Medicine, Department of Health, Animal Science and Food Safety, Large Animal Hospital, Reproduction Unit, 6 Via dell'Università, I-26900 Lodi, Italy
Keywords
Related Articles
- EP ID EP9276
- DOI -
- Views 372
- Downloads 28
Pregnancy diagnosis by laparoscopy in free range rhesus macaques (Macaca mulatta)
The present study involved 50 adult female rhesus macaques (Macaca mulatto) of age ranging between 4 to 15 years. Pregnancy diagnosis was done by using laparoscopic method. Anesthesia was achieved by using xylazine (2m...
Unusual haemodynamics in two dogs and two cats with portosystemic shunt - implications for distinguishing between congenital and acquired conditions
Extrahepatic porto-systemic shunt (PSS) in small animals can be congenital (CPSS) or acquired (APSS) as a consequence of portal hypertension (PH), and are distinguished on the bases of their anatomical pattern. A precis...
Liver abscesses in dromedary camels: Pathological characteristics and aerobic bacterial aetiology
The study was carried out at Nyala abattoirs, South Darfur State, Sudan during a period from 2009 to 2011. Slaughtered camels (822) were examined for pathological changes of liver abscesses and identification of the inv...
Student attitudes regarding the educational value and welfare implications in the use of model eyes and live dogs in teaching practical fundus examination: evaluation of responses from 40 students
This study sought to document student opinions on the educational value and welfare implications of use of artificial model eyes and live dogs in the training of veterinary students in examination of the canine fundus....
Longevity of Cane Corso Italiano dog breed and its relationship with hair colour
The Cane Corso Italiano belongs among the new dog breeds that were fully recognised by Federation Cynologique Internationale (FCI) in 2007. For the first time, this study describes a median lifespan using the data of 23...