FORMULATION OF KETOCONAZOLE LOADED NANO DISPERSIVE GEL USING SWOLLEN MICELLES TECHNIQUE AND ITS IN VITRO CHARACTERIZATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 3
Abstract
Objective: The objective of the present work was to formulate and characterize nano dispersive gel (NDG) for topical delivery of water-insoluble antifungal agent ketoconazole in order to enhance its solubility, penetration through the skin and antifungal activity.Methods: Nano dispersion of the drug was first prepared by swollen micelles technique (SMT) using tween 80 and chloroform which is then incorporated into the gel using carbopol 934. Ten formulations of ketoconazole loaded NDG was prepared and characterized for different physicochemical parameters like homogeneity, pH, spreadability, extrudability, practical yield, drug content, in vitro drug release, ex vivo permeation study, and biological parameter antifungal activity.Results: The formulated topical preparation exhibit pH in the range of 6.5 to 7.4, and unveiled excellent homogeneity, spreadability and extrudability. Out of 10 formulations, formulation F4 showed maximum drug content of 95.56±1.13% and practical yield of 97.23±0.51%. The in vitro drug release studies were performed using pH 7.4 phosphate buffer. Formulation F4 showed best in vitro drug release 96.52±0.52% at the end of 24 h of study. Ex vivo permeation study of formulation F4 carried out using franz diffusion cell, also manifested good permeation and flux of drug across the chicken skin. Antifungal activity test of formulation F4 was carried out by the cup plate method using Aspergillus niger strain against marketed ketoconazole unveiled higher antifungal activity than marketed one.Conclusion: The study confirmed formulation F4 to be an optimized and promising formulation for the effective treatment of topical fungal infections with enhanced solubility and penetration through the skin.
Authors and Affiliations
Mohammad Irshad Reza, Divya Goel, Rahul Kumar Gupta, Musarrat Hussain Warsi
Keywords
Related Articles
- EP ID EP567565
- DOI 10.22159/ijpps.2018v10i3.24552
- Views 97
- Downloads 0
EFFECTS OF STREPTOZOTOCIN INDUCED DIABETES MELLITUS TYPE 1 ON THE RAT BRAIN ANTIOXIDANT STATUS AND ACTIVITY OF ACETYL-CHOLINESTERASE: A NOVEL AND POTENTIAL TREATMENT BY VITEX NEGUNDO.
Objective: The objective of the present study was to determine the effect of streptozotocin (STZ) induced diabetes type 1 on activity of acetyl-cholinesterase (AChE) in rat brain and on the brain antioxidant status and a...
SEASONAL AND CIRCADIAN VARIATION OF BARBATUSIN IN SAMPLES OF PLECTRANTHUS GRANDIS WILLENSE GROWN IN NORTHEAST BRAZIL
Objective: This work includes the study of circadian and seasonal variation of barbatusin, active compound present in P. grandis (Plectranthus grandis), through the implementation and validation of an analytical method c...
EVALUATION OF TAXIFOLIN AND PHLORETIN AS ANTIANGIOGENIC: AN IN VIVO, IN VITRO EXPERIMENTAL ANALYSIS
Objective: To examine the antiangiogenic effect of flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chambe...
ANTIDIABETIC ACTIVITY OF LINALOOL AND LIMONENE IN STREPTOZOTOCIN- INDUCED DIABETIC RAT: A COMBINATORIAL THERAPY APPROACH.
Objective: To demonstrate the efficacy of a protein glycation inhibitor (limonene) and antihyperglycemic plant component (linalool) in combination towards better management of diabetesMethods: Antihyperglycemic activity...
CHEMOMETRIC SCREENING AND OPTIMIZATION OF LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF SEVEN ANTIHISTAMINES
Objective: Chemometric optimization and validation of a HPLC method for the simultaneous determination of seven antihistamines viz., loratadine, fexofenadine, desloratadine, levocetirizine, doxylamine, promethazine and c...