Free Radicals Scavenging and Protein Protective Property of Ocimum sanctum (L)
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 14, Issue 4
Abstract
Ocimum sanctum (L) is a well-known herbal plant used for the treatment of bronchitis, malaria, diarrhea, dysentery, skin disease, arthritis, eye diseases and insect bites. It is proposed to have anticancer, antidiabetic, antifungal, antimicrobial, cardio protective, analgesic and antispasmodic action. In our present study, we have tried to explore its antioxidant, free radical scavenging and its ability to prevent damage to protein from free radicals. The antioxidant and free radical scavenging activity of Ocimum sanctum leaves was determined using ferric thiocyanate, thiobarbituric Acid, 2, 2-diphenyl-1–picrylhydrazyl, hydrogen peroxide, hydroxyl radical and superoxide radical scavenging methods. Further, its protein protective property was evaluated using UV- VIS spectrophotometer methods. The results of ferric thiocyanate and 2, 2-diphenyl-1–picrylhydrazyl methods were determined that O. sanctum have strong antioxidant activity (92.12%) and free radicals scavenging property (50.74%) respectively. Further, hydrogen peroxide, hydroxyl radical and superoxide radical scavenging methods determined that extracts (100 µg/ml) have hydrogen peroxide (20.12%), hydroxyl radicals (12.68%) and superoxide radicals (21.68%) scavenging activity respectively. Hemoglobin is a standard protein which was damaged by hydrogen peroxide treatment and this damage was confirmed by UV-VIS spectrophotometer. The extract showed the significant pre and post protein protective action against hydrogen peroxide induced hemoglobin damage. Thus the study concluded that O. sanctum leaves have strong antioxidant, free radicals scavenging and pre and post proteins protective property.
Authors and Affiliations
Anand Kumar Keshari, Atul Srivastava, Akhilesh Kumar Verma, Ragini Srivastava
Keywords
Related Articles
- EP ID EP342280
- DOI 10.9734/BJPR/2016/31445
- Views 74
- Downloads 0
The Renal and Cardio Protective Effects of Aliskiren and Pentoxifylline Alone and in Combination on Streptozotocin Induced Diabetic Rats
Background/Aims: The aim of the present study is to investigate the renoprotective and cardioprotective effect of aliskiren and pentoxifylline, alone and in combination, on streptozotocin (STZ) induced diabetic rats. Met...
Tegumental Changes in Adult Schistosoma mansoni Induced by a New Imidazolidinic Derivative
Aims: Verify the potential of the schistosomicidal imidazolidine derivative (5Z)-3-(4-bromo-benzyl)-5-(4-chloro-benzylidene)-4-thioxo-imidazolidin-2-one. Study Design: In this study, we tested the imidazolidinic derivati...
Synthesis and Evaluation of Antimicrobial Properties of Some Chalcones
Introduction: Recently there has been a great deal of interest in the health benefits of phytochemicals, particularly prenylated and allylated flavonoids. Chalcones (1, 3-diaryl-2-propen-1-ones) and their derivatives are...
Influence of Process Parameters on Compressibility, Solubility and Release Characteristics of Melt Sonocrystallized Fenofibrate
Aims: The aim of the present study was to investigate the suitability of the melt sonocrystallization (MSC) technique to modify the processability properties along with solubility and drug release of anti-hyperlipidemic...
A Novel Approach for Studying Antitermite Efficacy of Different Leaf Extracts of Thevetia peruviana from Polluted and Non-polluted Sites
A novel method for evaluating antitermite activity was standardized whereby filter papers treated with 50% methanolic, 90% methanolic and aqueous extracts of Thevetia peruviana leaves from polluted and non-polluted sites...