Fructophilic lactic acid bacteria symbionts in honeybees – a key role to antimicrobial activities.
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 1
Abstract
Antibiotic resistance has emerged as an intensive subject for research to find an alternative tool against infections caused by pathogens with antibiotic resistance, Hence, a unique lactic acid bacteria (LAB) microbiota which is in symbiosis with honeybees was isolated in this study and was detected and identified by biochemical and molecular manners and its antibacterial activity was detected against pathogenic bacteria using the dual culture overlay assay. Results reveled isolation and identification of two genera: Fructobacillussp. (92.3%) and Lactobacillus sp. (7.7%) from a total of 38 isolate, these isolates were known as Fructophilic lactic acid bacteria (FLAB). The highest inhibition zone (37mm) produced by Fructobacillus fructosus(SHGH-1,SHGH-4, andSHGH-14) against Pseudomonas aeruginosaand (33mm) forFructobacillus fructosus(SHGH-11) against Staphylococcus aureus (MRSA). This study shows that FLABs are unique sources of antibacterial substances which is effective against pathogenic Gram positive and Gram negative bacteria
Authors and Affiliations
Shahad Mansour Salman, Ghadahmohammed Saleh
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