Gastro-duodenal protective effect of aqueous leaf extract of Daucuscarota sativus Linn. (Apiaceae) in rats and its possible mechanism of action
Journal Title: UNKNOWN - Year 2017, Vol 6, Issue 3
Abstract
Background: Daucuscarota sativus L. (Apiaceae) commonly known as ‘carrot’ is a multipurpose herb cultivated in different parts of the world for its edible roots, juice, oils and leaves. Carrot root as well as its leaves has been credited with many medicinal properties, including cleansing of the intestine and maintenance of acid-alkaline balance in the body. Carrot leaves also known as carrot tops or carrot greens have been used locally as a decoction for healing mouth sores, and in some cases, mixed with honey to clean festering wounds. The present study was therefore designed to evaluate the possible gastro-duodenal protective property of Daucuscarota sativus (DCS) aqueous leaf extract on laboratory-induced ulcers. Aqueous leaf extract prepared by maceration was orally administered one hour before ulcerogens in doses of 50, 100 and 200 mg/kg to groups of randomized adult rats of both sexes. Gastric ulcers were induced using pyloric ligation, methyleneblue, and acetic acid, while cysteamine, and indomethacin-histamine were separately employed for induction of duodenal ulcers in the animals. Positive controls were given standard drugs appropriate for each experimental model. Phytochemical screening of the freshly prepared extract was also carried out, as well as evaluating its antioxidant activity. In each of the models, the aqueous leaf extract of DCS showed a significant (p<0.05-0.001) dose-independent protection against peptic ulcer. The effects produced by the aqueous leaf extract of DCS were comparable to those of the standard drugs (omeprazole; 200 µg/kg, ranitidine; 50 mg/kg, and cimetidine; 50 mg/kg). Phytochemical analysis of the aqueous leaf extract of Daucuscarota sativus revealed the presence of flavonoids, tannins, alkaloids, and saponins, some of which have been reported to elicit cytoprotective effect. Antioxidant analysis showed significant scavenging effect of free radical using nitric oxide, lipid peroxidation and DPPH assay. The findings in this study suggest that the aqueous leaf extract of DCS possesses cytoprotective effect and also reduces secretion of secretagogues, thereby protecting against gastric and duodenal ulcers.
Authors and Affiliations
Esther Oluwatoyin Agbaje, Muyiwa Samuel Fageyinbo, Olaitan Oladele Alabi
Keywords
Related Articles
- EP ID EP451503
- DOI -
- Views 61
- Downloads 0
Pharmacovigilence practice for safety of medication system in India
Pharmacovigilance is very essential tool to ensure the safety of drug. It provides safety to patients in case of medication. Activity of pharmacovigilance is coordinates by National pharmacovigilance center in collaborat...
Cytotoxic activity of Acalypha indica L. hexane extract on breast cancer cell lines (MCF-7)
Cancer is the name given to a collection of related diseases. In all types of cancer, some of the body’s cells begin to divide without stopping and spread into surrounding tissues. Cancer can start almost anywhere in the...
Comparative effects of aqueuos extract of Phyllanthus amarus and its fractions on urinary excretion in rat
The aim of the present study was to compare the effect of Phyllanthus amarus extracts and its fractions on urinary excretion. Aqueous extract of Phyllanthus amarus was prepared by decoction of the whole plant and lyophil...
Cytotoxicological evaluation of semi-purified extracts of some dye yielding plants of the Kashmir Valley on Normal Intestinal Cell Line (IEC-6) by MTT assay
Plant extracts are widely used in many fields and there is a need to evaluate their cytotoxic effect to determine their non-cytotoxic concentration at which they can be used in a safe manner. Keeping this in view, the pr...
Standardization of Naga Bhasma prepared by two different Bhavana Dravya
Naga Bhasma is one of the herbo-metallic preparations used in Ayurveda and claimed to possess broad spectrum of therapeutic efficacy. The Standard manufacturing Procedures of Naga Bhasma prepared by two methods by changi...