Gene Xpert MTB/RIF Assay – An Effortless Tool for the Rapid Detection of Pulmonary and Extra-Pulmonary Tuberculosis with Detection of Rifampicin (RIF) Resistance
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 12
Abstract
Abstract: Tuberculosis (TB) is one of the major concerns of health policy and rapid detection of M. tuberculosis and detection of rifampicin (RIF) resistance in infected patients are essential for disease management. Multi-drug resistance (MDR) TB is defined as tuberculosis (TB) disease caused by a strain of Mycobacterium tuberculosis (MTB) that was resistant to at least isoniazid and rifampicin (RIF). The aim of this study was to evaluate the importance of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis (MTB) with detection of rifampicin (RIF) resistance. In this study 320suspected cases of pulmonary and extra-pulmonary TB were included. Their sputum and other (Pus, CSF, tissue, etc.,) samples were collected and subjected to smear microscopy test followed by GeneXpert MTB/RIF assay. Of total 320 suspected cases, Mycobacteria were detected in 152 patients (47.5%) by GeneXpert MTB/RIF and ZN stain. Among all, the highest detection rate of MTB was found by Genexpert 148(46.2%) followed by ZN smear 114(35.6%). Four ZN smear positive cases were negative by Genexpert MTB/RIF. However, RIF’s resistance was detected in only 08 cases (5.4%) by GeneXpert MTB/RIF. GeneXpert MTB/RIF assay is efficient and reliable technique for the rapid diagnostic of TB. The GeneXpert MTB/RIF assay is a simple, high sensitivity and specificity for RIF resistance detection implies for diagnosis of MTB and RIF resistance in MDR cases as well as suspected smear negative and extra-pulmonary cases.
Authors and Affiliations
Dr. Sanjeev Kumar, Mr. Rambabu Sharma, Dr. SadhanaShanker .
Keywords
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