Genetic analysis of a weak D type61 sample from a blood donor, Jiangyin
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 7
Abstract
Objective To genetically analyze the Del sample from a blood donor in Jiangyin and make clear the molecular basis of the serological phenotype. Methods The EDTA anticoagulant blood were collected: buffy coat were used for nucleic acid extract and cDNA analysis; red blood cells for serological test. Tube method and microcolumn gel were used for serological test. Genotyping kit were used for exon analysis. Gene mutation was analyzed using the sequence analyzer. Results Serological analysis demonstrated the sample′s RhD phenotype was Del. The phenotype of RhCE was CCEe. Real-time fluorescence quota PCR result demonstrated the existence of all exones. Weak D15 and RHD*DEL1 [RHD(1227G>A)], which had a high frequency of occurrence in China, were excluded according to real-time fluorescence quota PCR result. Sequence analyzing result verified RHD(28C>T) SNP mutation in cDNA. The genotype of this sample was RHD*01 W. 61[RHD(28C>T)]. Conclusion A weak D61 was found among blood donors in our city, Jiangyin.
Authors and Affiliations
Fang WANG, Mengyao BIAN, Qiurong YU, Minglei WU, Haiping ZHAO, Ling SUN, Buqiang WANG, Hongjun GAO, Haicai SHI, Yi WU, Ming GAO, Yuping CHEN
Keywords
Related Articles
- EP ID EP738285
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.07.005
- Views 32
- Downloads 0
Blood security and management of transfusion-dependent thalassaemia (TDT) patients in Nanning: analysis of " collection-based-supply" blood management mode
Objective To retrospectively analyze the blood use of transfusion-dependent thalassemia (TDT) patients in 9 designated transfusion medical institutions from 2018 to 2023 in Nanning, and to evaluate the effect of " three...
The clinical study of thromboelas-tography combined with coagulation four items and platelet count to guide platelet transfusion in critically ill patients
Objective To explore the clinical value of thromboelas-tography, coagulation four items and platelet count in guiding platelet transfusion in critically ill patients. Methods A total of 188 critically ill patients in Int...
Distribution frequencies of KIR genes among the Korean, Manchu and Han ethnic groups in Jilin Province
Objective To provide foundational data for exploring the association between KIR genes and diseases by analyzing the frequency and polymorphism of killer-cell immunoglobulin-like receptor (KIR) genes in Han, Manchu and K...
Study on the distribution of ABO blood group in patients with pancreatic cancer
Objective To investigate whether there is a correlation between the differences in ABO blood group distribution in patients with pancreatic cancer, and to evaluate the relative risk. Methods Patients with pathological di...
Blood transfusion strategies in patients on extracorporeal membrane oxygenation
Objective To analyze the blood transfusion strategies in extracorporeal membrane oxygenation (ECMO) recievers in our hospital, so as to explore the clinical application and efficacy of blood transfusion in patients on EC...