GENETIC AND BIOCHEMICAL PECULIARITIES OF SOYA MUTANT LINES
Journal Title: Вісник Одеського національного університету. Біологія - Year 2018, Vol 23, Issue 2
Abstract
The aim of the work was to study genetic and biochemical peculiarities of soya lines, obtained after treatment of seeds with chemical mutagens. In order to realise the stated aim the following tasks were set: 1) to determine population-genetic indices of soya samples, 2) to establish genetic distances between lines and parental material, 3) to determine potential correlative relationship between loci of the enzymes studied and other genetic systems, as well as morphological and physiological parameters. Material. The lines, obtained by chemical mutagenesis of Femida variety. Soya varieties Femida and Korada. Methods. Electrophoresis. Allozyme analysis. Enzymes of exchange of active oxygen forms: NADP N-oxydase, peroxydase and superoxyzedismutase were used as protein markers. Methods of population genetics. Correlation analysis. Methods of descriptive statistics. Computer data processing. Results. With respect to the loci studied the soya samples demonstrated considerable polymorphism, constituting 79 %.Considerable genetic heterogeneity of the soya samples was determined as to gene-enzyme systems. Wright and Gst-statistics showed that genetic heterogeneity is connected both with individual and with internal- and inter-group variability. Contribution of various types of variability into general differentiation was approximately equal. Inter-grout variability was manifested in form on different occurrence of different alleles of enzyme loci and different frequency of alternative genotypes. In addition to quantitative change of genotype frequency, other forms of genotype composition transformation were observed. So, fraction of heterozygous genotypes by loci NPOX-3, -4, -5, as well as SOD-3 increased in mutant lines in comparison with parental form. On the contrary, frequency of heterozygotes decreased in loci POX-3 and POX-6. Inversion of frequencies of homozygotes by alternative alleles was observed in locus POX-6 in mutant forms in comparison with output variety. The described changes of genotypic composition also observed in comparison of the studied varieties – Femida and Korada. On the whole, varieties Femida and Korada were different from each other by 10 of 15 polymorphic loci (67 %). Line M29 turned out to be more differentiated with considerable contribution to the general heterogeneity of individual variability. With considerable genetic heterogeneity the average heterozygosity by the studied loci was inconsiderable for the entire totality of samples and constituted 7 %. It was shown that genetic distances between different groups of soya are within intraspecific limits in spite of high level of heterogeneity. Basing on Neya index line M323 was more remote from parental form than sisterly mutant line. Reliable correlative relationship between some loci of izozymes and some economically valuable features of soya, as well as photosensitivity genome E7 were determined. The strongest interrelationship was observed for peroxidise and superoxidedismutase, which can testify to an important role of the said enzymes in regulation of plant development processes.
Authors and Affiliations
V. A. Toptikov, D. A. Zharikova, H. A. Chebotar, I. V. Temchenko, S. V. Chebotar
Keywords
Related Articles
- EP ID EP504139
- DOI 10.18524/2077-1746.2018.2(43).147013
- Views 60
- Downloads 0
STATUS OF BIOELECTIC MYOCARDIAL ACTIVITY IN 400 M DISTANCE RUNNERS DEPENDING ON THE SEX
Introduction. The method of electrocardiography deeply reflects the essence of bioelectric processes in myocardium, characterises deviation from the norm state, demonstrating locality and specificity of pathogenic change...
MICROBAL GROUPS AND BIOLOGICAL ACTIVITY OF THE ROOT ENVIRONMENT IN PHLOX PANICULATA L.
The study of soils, in particular their allelopathic and microbiological features, remains an important but seldom investigated aspect in the cultivation of Phlox paniculata L. The microbial groups of the root soil and i...
CHARACTERISTICS OF THE INITIAL STAGE OF VEGETATION DEMUTATION IN FALLOWS UNDER THE DONETSK REGION
The article considers the changes in species composition of herbage and projective cover in the areas taken out of agricultural use of various ages. The ecological certification (by the method of L. Belgard), makes it po...
ANTIMICROBIAL ACTION OF LED RADIATION ON CAUSATIVE AGENTS OF OPPORTUNISTIC INFECTIONS
The antibiotic resistance of microorganisms-causative agents of opportunistic infections significantly reduces the effectiveness of treatment of diseases caused by Pseudomonas aeruginosa and Candida albicans. The aim of...
CORRELATION BETWEEN ANTHROPOMETRIC AND ELECTROMYOGRAPHIC INDICES IN PHYSICALLY TRAINED PEOPLE
Introduction. It is known that the results of competitions of athletes are influenced by their anthropometric indices. However, the concept of physical development includes both a set of anthropometric indicators and par...