GENETIC AND BIOCHEMICAL PECULIARITIES OF SOYA MUTANT LINES
Journal Title: Вісник Одеського національного університету. Біологія - Year 2018, Vol 23, Issue 2
Abstract
The aim of the work was to study genetic and biochemical peculiarities of soya lines, obtained after treatment of seeds with chemical mutagens. In order to realise the stated aim the following tasks were set: 1) to determine population-genetic indices of soya samples, 2) to establish genetic distances between lines and parental material, 3) to determine potential correlative relationship between loci of the enzymes studied and other genetic systems, as well as morphological and physiological parameters. Material. The lines, obtained by chemical mutagenesis of Femida variety. Soya varieties Femida and Korada. Methods. Electrophoresis. Allozyme analysis. Enzymes of exchange of active oxygen forms: NADP N-oxydase, peroxydase and superoxyzedismutase were used as protein markers. Methods of population genetics. Correlation analysis. Methods of descriptive statistics. Computer data processing. Results. With respect to the loci studied the soya samples demonstrated considerable polymorphism, constituting 79 %.Considerable genetic heterogeneity of the soya samples was determined as to gene-enzyme systems. Wright and Gst-statistics showed that genetic heterogeneity is connected both with individual and with internal- and inter-group variability. Contribution of various types of variability into general differentiation was approximately equal. Inter-grout variability was manifested in form on different occurrence of different alleles of enzyme loci and different frequency of alternative genotypes. In addition to quantitative change of genotype frequency, other forms of genotype composition transformation were observed. So, fraction of heterozygous genotypes by loci NPOX-3, -4, -5, as well as SOD-3 increased in mutant lines in comparison with parental form. On the contrary, frequency of heterozygotes decreased in loci POX-3 and POX-6. Inversion of frequencies of homozygotes by alternative alleles was observed in locus POX-6 in mutant forms in comparison with output variety. The described changes of genotypic composition also observed in comparison of the studied varieties – Femida and Korada. On the whole, varieties Femida and Korada were different from each other by 10 of 15 polymorphic loci (67 %). Line M29 turned out to be more differentiated with considerable contribution to the general heterogeneity of individual variability. With considerable genetic heterogeneity the average heterozygosity by the studied loci was inconsiderable for the entire totality of samples and constituted 7 %. It was shown that genetic distances between different groups of soya are within intraspecific limits in spite of high level of heterogeneity. Basing on Neya index line M323 was more remote from parental form than sisterly mutant line. Reliable correlative relationship between some loci of izozymes and some economically valuable features of soya, as well as photosensitivity genome E7 were determined. The strongest interrelationship was observed for peroxidise and superoxidedismutase, which can testify to an important role of the said enzymes in regulation of plant development processes.
Authors and Affiliations
V. A. Toptikov, D. A. Zharikova, H. A. Chebotar, I. V. Temchenko, S. V. Chebotar
Keywords
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- EP ID EP504139
- DOI 10.18524/2077-1746.2018.2(43).147013
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