THE EFFECT OF PLATELET-RICH AND PLATALET-POOR PLASMA ON METABOLIC PROFILE OF AUTOLOGOUS HUMAN MONOCYTES IN VITRO
Journal Title: Вісник Одеського національного університету. Біологія - Year 2017, Vol 22, Issue 1
Abstract
The aim of the study was to perform the comparative investigation of the effect of platelet-rich (PRP) and platelet-poor (PPP) plasma on autologous human monocyte metabolism in vitro. Adult healthy 35–40 year old men were recruited to participate in the study. To characterize monocyte metabolism intracellular production of reactive oxygen species (ROS) and phagocytic activіty were determined by flow cytometry, with nitrite production being determined in Griess reaction and arginase activity - in colorimetric assay. The obtained results suggest that treatment of monocytes with autologous L-PRP and PPP preparations causes their anti-inflammatory metabolic activation.
Authors and Affiliations
A. V. Rakukha, Y. L. Holiuk, T. Y. Pshenychnyi, T. S. Maslova
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