THE EFFECT OF PLATELET-RICH AND PLATALET-POOR PLASMA ON METABOLIC PROFILE OF AUTOLOGOUS HUMAN MONOCYTES IN VITRO
Journal Title: Вісник Одеського національного університету. Біологія - Year 2017, Vol 22, Issue 1
Abstract
The aim of the study was to perform the comparative investigation of the effect of platelet-rich (PRP) and platelet-poor (PPP) plasma on autologous human monocyte metabolism in vitro. Adult healthy 35–40 year old men were recruited to participate in the study. To characterize monocyte metabolism intracellular production of reactive oxygen species (ROS) and phagocytic activіty were determined by flow cytometry, with nitrite production being determined in Griess reaction and arginase activity - in colorimetric assay. The obtained results suggest that treatment of monocytes with autologous L-PRP and PPP preparations causes their anti-inflammatory metabolic activation.
Authors and Affiliations
A. V. Rakukha, Y. L. Holiuk, T. Y. Pshenychnyi, T. S. Maslova
Keywords
Related Articles
- EP ID EP311336
- DOI 10.18524/2077-1746.2017.1(40).96527
- Views 90
- Downloads 0
DYNAMICS OF THE CONTAMINATIONS WITH FEATHER MITES (ACARI: ASTIGMATA) OF BLACKCAP AND ROBIN MIGRATING THROUGH ZMIINYI ISLAND (THE BLACK SEA, UKRAINE).
Problem. The contamination of the plumage of the Blackcap Sylvia atricapilla (Sylviidae) and the Robin Erithacus rubecula (Muscicapidae), migrating through Zmiinyi island (the Black Sea, Ukraine) with feather mites with...
THE INFLUENCE OF ANTIOXIDANT COMPOUNDS ON THE BIOCHEMICAL INDICATORS OF RATSʼ BLOOD SERUM UNDER ACUTE HEPATITIS
Introduction. Hepatitis of various etiologies is a global problem, which is caused by their high socio-economic significance and widespread prevalence among people of working age. One of the actual problems is the search...
ANTIMICROBIAL ACTION OF LED RADIATION ON CAUSATIVE AGENTS OF OPPORTUNISTIC INFECTIONS
The antibiotic resistance of microorganisms-causative agents of opportunistic infections significantly reduces the effectiveness of treatment of diseases caused by Pseudomonas aeruginosa and Candida albicans. The aim of...
ISOLATION OF MATRIX METALLOPROTEINASE-2 FROM NONMALIGNANT BR EAST TISSUES IN WOMEN
Matrix metalloproteinase - 2 (ММР-2) is zinc- and calcium-dependent extracellular proteinase which takes part in reorganizations of extracellular matrix in many physiological and pathological processes. The research aim...
LICHENS AND LICHENICOLOUS FUNGI OF NATIONAL NATURE PARKS «NYZHNOSULSKY» AND «PYRYATYNSKY»
Abstract Introduction. Currently, the protected areas of the Poltava region remain poorly understood. Purpose. This is a comprehensive study of the lichens and lichenicolous fungi on the territory of two National Nat...