Genetic Diversity among Clinical Isolates of Helicobacter Pylori Detected From India
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 1
Abstract
Background: Helicobacter pylori is a pathogenic gram negative bacteria found in human stomach. It is present in almost 50% of human population worldwide and its infection is linked to development of various diseases ranging from gastrointestinal disorders to gastric cancer. The objective of the present study was to assess the epidemiology and genomic variability of H. pylori strains prevalent in patients of Jammu (J&K). Methods: Mix of qualitative case study methods (key informant interviews, document review and structured questionnaires) was used to study epidemiology. Clinical biopsy specimens were collected from the patients undergoing gastrointestinal endoscopy. The DNA diversity was determined by RAPD and multiplex PCR. Results: 40 patients were found H. pylori positive out of which 64% were males and 36% were females. Subjects who were in the age group of 36-45 years, smokers, alcohol consumers, non-vegetarians were found to be associated with this infection. Abdominal pain was the most frequent symptom. Primer 1283 was more sensitive compared to 1254 and 1281 in differentiating infections in our patients. Most of the patients were cagA positive. Conclusions: The preliminary results obtained in this study indicate that RAPD markers may show requisite polymorphism in order to assess the Genetic variability among various H. pylori strains. Detailed characterization of virulence genes and their allelic types may prove highly beneficial for eradication therapies of H. pylori.
Authors and Affiliations
Shelly Sehgal, Sachin Gupta, Sanjana kaul, Manoj K Dhar
Keywords
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- EP ID EP541652
- DOI 10.21276/ijmrp.2017.3.1.017
- Views 69
- Downloads 0
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