Study of Haemoglobinopathies and Hb Variants in Ajmer Region, Using HPLC
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 4
Abstract
Introduction: Haemoglobinopathies are the most common hereditary disorders, characterized by abnormalities both quantitative and qualitative in the synthesis of haemoglobin and pose a major health problem in India. Aims & Objectives: Haemoglobinopathies were studied in relation to age, sex, ethnic group, red blood cell indices and peripheral blood smear examination, using HPLC. Materials & Methods: A total of 717 patients of suspected haemoglobinopathies were analysed on the Bio-Rad Variant HPLC system by β-thal short program. The retention times, proportion of the haemoglobin (%) and the peak characteristics for all haemoglobin fractions were recorded. The blood samples of patients was analysed for complete haemogram on Auto Haematology Analyser. Results: A total 717 cases were studied. Of these, 395 (55.09%) had normal and 322 (44.91%) showed abnormal haemoglobin fractions on HPLC. Out of 322, 178 cases (55.28%) were males and 144 cases (44.72%) were females. Of these, most common haemoglobinopathy was β-Thalassemia Minor/BTT 206 cases (28.73%) followed by β-Thalassemia Major 43 cases (5.99%), δβ-Thalassemia 21 cases (2.93%) and HPFH (Hereditary persistence of fetal haemoglobin) 14 cases (1.95%). Other hemoglobinopathies were also identified in smaller proportions. Prevalence of haemoglobinopathies were higher in the Sindhi community of Ajmer region (Raj.) India. Conclusion: HPLC is very simple, rapid, accurate and superior technique in early detection of various haemoglobin disorders.
Authors and Affiliations
Geeta Pachori, Radha Kishan Meena, Ravi Kant Sunaria, Pramendra Pachori, Tushar Bayla
Keywords
Related Articles
- EP ID EP546734
- DOI 10.21276/ijmrp.2017.3.4.023
- Views 86
- Downloads 0
Chronic Interstitial Nephritis Induced by Deferasirox (Exjade): An Oral Iron-Chelating Agent in a Patient with Thalssemia Major
We report on the development of chronic interstitial nephritis induced by Deferasirox (Exjade). The patient is a 40-year-old woman, with history of thalassemia major had developed progressive increase in serum creatinine...
Ordinarily Isolated Organisms from Blood Cultures of Neonates and Children: A Hospital Based Study
Background: Sepsis is a serious medical condition caused by an overwhelming immune response to infection. Neonatal sepsis specifically refers to the presence in a newborn baby of a bacterial blood stream infection (BSI)...
Patau Syndrome Survival 6 Years & It’s Associated Ocular Findings: Case Report
Trisomy 13 is a rare syndrome with a prevalence of 1:12000- 1:29000 in newborns. Eighty-two percent die within one month. The majority of patients with patau syndrome have several ophthalmic abnormalities including micro...
Assessment of Outcome and Complications of Proximal Femoral Nail in The Management of Intertrochanteric Fractures
Introduction: Years ago, intertrochantric fractures often used to be a terminal event in the life of feeble and fragile patients. The basic problem was not of union, but of complications arising due to immobilisation in...
The Prevalence of Learning Difficulties and Its Academic Impact Among Elementary School Students in Riyadh, Saudi Arabia
Introduction: Learning Difficulty (LD) is a disorder in one or more of basic psychological processes involved in understanding or using language spoken or written, that may manifest itself on student’s academic performan...