Genetic Diversity and Distribution of Cassava Brown Streak Virus and Ugandan Cassava Brown Streak Virus in Major Cassava-growing Regions in Kenya
Journal Title: Annual Research & Review in Biology - Year 2016, Vol 10, Issue 5
Abstract
Cassava brown streak disease (CBSD) is caused by two viruses; Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) that are genetically distinct. The disease can cause losses of up to 100 million US Dollars and it has a big impact on cassava yields. There is relatively little genetic information of CBSVs, their distribution and genetic diversities in Kenya. This study was therefore to establish the extent of distribution and diversity of CBSV and UCBSV strains in the Kenya. A survey was conducted in Coast, Western and Nyanza which are the major cassava-growing areas of Kenya. The mean disease incidence in Coast was 47.8%, Western 33.0% and Nyanza 55.9%. The average mean severity score cassava brown streak virus was 2.0 for Coast, 2.4 for Western and 2.6 for Nyanza. A reverse transcriptase polymerase chain reaction (RT-PCR) was used to screen 131 samples and of these 24 positive and negatives samples representing each region were selected for next generation sequencing. RT-PCR detected single infections of CBSV, UCBSV and co infections 19.8%, 22.1% and 34.4% of the positive reactions respectively countrywide. Alignment from assembled coat protein sequences revealed two clusters representing the two strains. The study also revealed a mixed infection in the samples from the surveyed fields. The widespread distribution of CBSV and UCBSV in Coast, Western and Nyanza farmer’s field in Kenya has implications in the cassava production in those regions.
Authors and Affiliations
T. M. Kathurima, A. B. Nyende, S. M. Kiarie, E. M. Ateka
Keywords
Related Articles
- EP ID EP351043
- DOI 10.9734/ARRB/2016/26879
- Views 85
- Downloads 0
Molecular Characterization of Diverse Genotypes of Indian Bread Wheat (Triticum aestivum L. Em. Thell) by Using SSRs Markers for Leaf and Stripe Rust Resistance
Wheat (Triticum spp) is a crop of global significance and occupies a premier place among cereals. Due to its high nutritive value and huge acreage devoted for cultivation of wheat, it is a staple food supplying approxima...
‘Synbiofilm’- A Friendly Microbial Association in Aquatic Ecosystem
‘Biofilm’ is a multi-community microbial association living on submerged substrates. Using the online search engine www.OJOSE.com to identify the 100 most recent journal articles, we found that the term ‘biofilm’ had bee...
Molecular Method for Direct Detection of Brucella spp. in Human Blood Samples
Background and Aim: Brucellosis is a zoonosis of worldwide public health and economic importance, affects mainly domestic animals and it may be the genus Brucella, transmitted to humans by contact with infected domestic...
Death Feigning Behaviour of Several Frog Species from Kedah, Peninsular Malaysia
Two individuals of ranid species, Hylarana picturata and Hylarana laterimaculata, were collected from Sungai Sedim Recreational Forest. A single individual of dicroglossid species, Occidozyga laevis and two individuals o...
Preliminary Report on Fusarium oxysporum f. sp. lycopersici (Sensu lato) From Some Tomato Producing Agroecological Areas in Southwestern Nigeria and Susceptibility of F1-Resistant Tomato Hybrid (F1-Lindo) to Infection
Aims: To isolate Fusarium oxysporum f.sp. lycopersici from infected tomato plants in selected tomato producing agroecological areas in Southwestern Nigeria and evaluate susceptibility of F1-resistant tomato hybrid (F1-Li...