Genetic diversity assessment in nine cultivars of Catharanthus roseus from Central Gujarat (India) through RAPD, ISSR and SSR mar
Journal Title: Journal of Research in Biology - Year 2011, Vol 1, Issue 8
Abstract
The genetic relationship study was carried out among nine different cultivars of Catharanthus roseus using RAPD, ISSR and SSR markers. In RAPD analysis, out of twenty primers, six primers amplified 592 bands out of which 466 were polymorphic while rest was monomorphic. This gave high (78.71%) polymorphism among nine cultivars. In ISSR analysis, 78.94% polymorphism was observed, while in SSR analysis, 76.62% polymorphism was observed. The dendogram based on all three markers separated the cultivars in two major groups. ‘Blue pearl’, ’cooler red’, and ‘pacifica apricot’were in the same group, while rest was in other group. Amongst all ‘Pacifica liac’ and ‘Albus with red eye’ were found to be closely related. Our results showed that all RAPD, ISSR and SSR markers are sensitive and effective tool for genomic analysis in Catharanthus roseus. This study provides key platform for further crop improvement and cross breeding
Authors and Affiliations
Sanjay Lal, Kinnari N. Mistry,, Smit. D Shah, Riddhi Thaker, Parth B. Vaidya
Keywords
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