Genetic polymorphism in Lens culinaris collected from malakand division Khayber Pakhtunkhwa, Pakistan
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2016, Vol 8, Issue 2
Abstract
A major part of the human di et al over the world consists of cereals and legumes. Legumes are however, considered as the major source of protein and dietary amino acid for man and farm animals. The detailed evolutionary relations and cluster analysis in this group of plants may help in further manipulations and modifications of plants leading to enhanced nutritional supplementation for consumption. To estimatethe picture of genetic diversity in lentil (Lens culinaris)the proteomics assay was carried out. A total of 60 cultivars were collected from 60 lentil growing areas of Malakand Division Khyber Pakhtunkhwa, Pakistan. The obtained lines were biochemically evaluated for the purpose to check the genetic heterogeneity. Based on proteomics homology dendrogram and cluster plottingsorted 60 cultivars into 4 clusters i.e. Cluster-1 (sorted forty cultivars), cluster-2 (grouped single cultivar), cluster-3 (had seventeen genotypes) and cluster-4 (grouped two cultivars).Thirteen loci (bands) were spotted in the collected germplasm of lentils through SDS-PAGE. Intra species locus contribution toward genetic diversity (LCTGD) was 84.61. Out of thirteen loci, locus-1, 2, 3,4,5, 6,7,8, 9, 10 and 11 showed polymorphic.The results showed that SDS-PAGE displayed a low level Genetic polymorphism in our accessions but higher than in other legumes, and it can be increased by the addition of more extensively sourced germplasm.
Authors and Affiliations
Syed Fazal Wadood, Nazim Hassan, Anela Khaliq, Nausheen,Tour jan, Abdul Ghafoor, Murad Khan, Mohammad Nisar
Keywords
Related Articles
- EP ID EP38728
- DOI -
- Views 151
- Downloads 0
Evaluation of some ecological factors, physiological and morphological traits of Salvia tebesana Bunge
Study on relationships between a species with its surrounding biotic and abiotic environment provides valuable information in terms of optimum choice for utilization, propagation, breeding and domestication. For this pur...
Investigation of otolith in some species of ariidae in persian gulf and Oman Sea
This study aimed to investigation of otolith in some species of Ariidae family in Persian Gulf and Oman Sea. Sampling lasted from September 2011 to December 2012. During this period two species (Arius dussumieri and Ar...
Population density of Aphis fabae Scopoli (Hemiptera, Aphididae) and its natural enemies in intercropping of faba bean (Vicia faba L.) and dragonhead (Dracocephalum moldavica L.)
Increasing crop diversity via intercropping is a simple and effective strategy for reducing pest density. A field experiment was conducted at Research Farm of Tabriz University, Tabriz, Iran during 2014. The experiment...
Tumor necrosis factor alpha in smokers and non-smokers
Previous investigations have described a positive association between smoking and systemic inflammation. To compare serum Tumor necrosis factor alpha (TNF-α) between smoker and non-smoker men, fifteen healthy adult smo...
A survey on influence of summer monsoon on diversity and density of bivalves in the Iranian Coasts of Oman Sea
Seasonal winds (Monsoon) significantly affect the ecological and environmental characteristics of Oman Sea and its sea-bed; in fact monsoon, being the obvious seasonal feature of the area, are the major factor in changi...