Genetic transformation by Agrobacterium tumefaciens of embryogenic cell suspensions of banana cultivar Grandenaine (AAA)
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 1
Abstract
The black Sigatoka (Mycosphaerella fijiensis Morelet) has become in the last years, the most destructive disease that affects the production of banana and plantains world-wide. The present work was made with the objective to obtain transgenic plants of banana cultivar Grand naine (AAA) resistant to this disease with the use of genetic transformation. Embryogenenic cell suspensions obtained from somatic embryos formed from immature male flowers, were used for the transformation by Agrobacterium tumefaciens. The bacterial strain EHA-105 was used with the binary plasmids pHCA-58, pHCG-59 and pHGA-91, which contain different combinations of genes that encode for the antifungal ® chitinase, glucanase enzymes and the AP-24 osmotin. The commercial herbicide BASTA was used as selective agent. One hundred ten putative transformed lines of the three constructions were obtained, after three selection months in the culture medium. The transgenic events were verified by means of Polymerase Chain Reaction analysis.
Authors and Affiliations
Idalmis Bermúdez, Rafael Gómez, Borys Chong, Maritza Reyes, José M. Machado, Orelvis Portal, Bárbara Ocaña, Yelenys Alvarado, Michel Leiva, Mayra Acosta, Mileidy Cruz, Belkis Roque, Rony Swennen , Laszlo Sagi, Lázaro Hernández
Keywords
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