Genome sequencing and analysis of BCG strain for vaccine production in China
Journal Title: Shanghai Journal of Preventive Medicine - Year 2023, Vol 35, Issue 3
Abstract
ObjectiveTo conduct the sequencing and preliminarily analysis of the whole genome of BCG Shanghai D2PB302 strain ï¼hereinafter referred to as BCG Shanghai D2 strainï¼ï¼ which has been used exclusively for the vaccine production in China.MethodsThe DNA of of BCG Shanghai D2 strain ï¼D2-JIA12-1ï¼ was extractedï¼ and the whole genome was sequenced by Pacbio-RS â ¡. The sequence data was assembled by Smrtlink and polished with the illumina data. Genesï¼ tRNA and rRNA were predicted based on the sequence data. The functional annotation of predicted genes was performed through BLASTP. The IVE-TB antigen gene and MTBVAC were selected as the target sequences to be compared with Mycobacterium tuberculosis H37Rv ï¼NC_000962.3ï¼.ResultsThe sequence length of BCG Shanghai D2 strain was 4 045 232 bpï¼ and the GC content was 65.66%. A total of 4 259 protein-encoding genes were predictedï¼ with an average gene size of 933 bp. 2 476 genes had biological functions and others were hypothetical proteins.144 virulence genes were obtained by comparing with the VFDB. There were 29 type â ¦ secretion system genes and 10 PE/PPE protein family genes.ConclusionThe whole genome sequence of BCG Shanghai D2 strain is clarified. It lays a broad foundation for subsequent detection of the stability of major antigen genes.
Authors and Affiliations
LIU Zhaoyang,ZHU Yongqiang,XU Yingmei,ZHAO Yueye,ZHU Tiaoxia,MA Leijun,CHEN Zhewen,
Keywords
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