Genotoxic Study of Chewing Leaf Tobacco in Swiss Albino Mice
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 1
Abstract
The tobacco plant Nicotiana has probably been responsible for more deaths than any other herb as it is market driven commodity of economic benefit. While the majority will likely be killed by use of cigarettes, tobacco use in other forms also contributes to worldwide morbidity and mortality. Chewing leaf tobacco is less used now as the ban is imposed on it. We tested the genotoxic potential of chewing leaf tobacco using in vivo cytogenetic tests- peripheral blood micronucleus test, and sperm abnormality assay. Three doses of tobacco viz., 3%, 5%, and 10% were given for 14 days. Cyclophosphamide, an indirect acting clastogen was used as positive control agent and it was injected intra peritoneally to the animals only once. Double distilled water was used as negative control. The frequency of micronucleated normochromatic erythrocytes (MNNCE) was increased in tobacco treated mice with the maximum MN being induced in NCEs at 10% dose. All three tobacco doses used in this study, induced significant abnormal sperms compared to controls (P<0.05). The chewing leaf tobacco at a certain concentration is genotoxic.
Authors and Affiliations
Ashoka CH, Mohammed S Mustak
Keywords
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- EP ID EP670637
- DOI 10.21276/ijlssr.2018.4.1.19
- Views 87
- Downloads 0
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