GENOTYPIC AND BIOCHEMICAL CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM SHEEP AT SLAUGHTER IN AMMAN-JORDAN
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 4
Abstract
Pasteurella multocida (p.m.) is a gram negative coccobacilli bacterial pathogen, classified into three subspecies, five capsular serogroups, which is the causative agent of a range of diseases in domestic animals of economic significant worldwide. The aims of this study are isolation of P. multocida from sheep and identification by biochemical tests and polymerase chain reaction technique (PCR) technique. Seventy samples were isolated from sheep lung lesions and were cultured for P. multocida using conventional method and isolated colonies were subjected to biochemical tests and for identification by PCR. The results in the present study showed that the biochemical tests provided an approximate 99.9% (an excellent identification for P. multocida). The PCR analysis applied on direct bacterial colonies using specific primer gave an amplified product (219 bp) specific for P. multocida, this result confirmed the results of biochemical tests and provided obvious results for the identification of this bacteria using the specific primer PMout for omp gene of P. multocida. The PCR assay was found to provide rapid and accurate diagnosis of P. multocida isolates obtained from sheep respiratory infections during the investigation of suspected hemorrhagic septicemia outbreaks. PCR technique is recommended to be used for identification procedure to be applicable in all veterinarian laboratories for bacterial identification; likewise the production of veterinary vaccines productions companies. In addition RAPD technique is reliable means of discriminating between P. multocida isolates obtained from sheep.
Authors and Affiliations
Khaled Al-Kubaisy| Biomedical Science Department, Al-Balqa Applied University, Zarqa College, Zarqa-Jordan, Maissa Shawagfeh| Biomedical Science Department, Al-Balqa Applied University, Zarqa College, Zarqa-Jordan, Faed Asfour| Biotechnology Department, Al-Balqa Applied University, Salt-Jordan, and Sabah E. Elmohtaseb| Biomedical Science Department, Al-Balqa Applied University, Zarqa College, Zarqa-Jordan
Keywords
Related Articles
- EP ID EP3012
- DOI -
- Views 349
- Downloads 21
Serofrecquancy of Human Immuno Deficiency virus among Known hepatitis Bvirus infected patients attending Talodihospital, SouthkordofanState, Sudan
Background: Co infection with human immuno deficiency virus (HIV) and hepatitis B virus (HBV) is common; worldwide, an estimated 10% of HIV-infected persons have chronic hepatitis B; because The incidence of traditional...
DETECTION OF GENETIC POLYMORPHISM OF NAD (P) H: QUINONE OXIDOREDUCTASE 1 (NQO1) AMONG SUDANESE PATIENTS WITH ACUTE LYMPHOID LEUKAEMIA
Polymorphic variations of several genes associated with dietary effects and exposure to environmental carcinogens may influence susceptibility to leukemia development. This study aimed to examine the association of NQO1...
PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BACTERIA CAUSING POSTOPERATIVE WOUND INFECTIONS IN PORT-SUDAN
Background Postoperative nosocomial infections remain a major problem in health care facilities, If not prevented it will lead to an extended length of stay, high excess of cost and high morbidity and mortality. Aim: T...
CLINICAL EVALUATION OF AN IN-OFFICE APPLICATION OF TWO COMMERCIALLY AVAILABLE DENTIFRICES ON DENTINE HYPERSENSITIVITY IN CHRONIC PERIODONTITIS PATIENTS
Aim: To evaluate the clinical effectiveness of an in-office application of desensitizing paste containing 8% arginine calcium carbonate and 5% potassium nitrate paste on dentine hypersensitivity in chronic Periodontitis...
NUTRIENT INTAKE AMONG SCHOOL GOING ADOLESCENT GIRLS IN LUCKNOW DISTRICT, INDIA
Background: Adolescent girls form an important vulnerable sector of population. Under-nutrition among adolescents is a serious public health problem internationally, especially in developing countries. Aim: To assess n...