Genotypic and Phenotypic Detection of Certain Virulent Determinates of Entrococcus Faecalis Associated With Dental Caries- an in vitro study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 12
Abstract
Enterococci have transformed over the past century from being an intestinal commensal organism of little clinical importance to becoming the second most widespread nosocomial pathogen and is related with considerable mortality and morbidity. Considering its multitude of resistance to different infections with high grade severity, we have attempted this study. A sum of 20 E. faecalis isolates from patients with dental caries were subjected to antibiotic sensitivity test followed by the detection of hemolysin and gelatinase enzyme activities and determination of ace and efaA genes encoding forcollagen binding protein and endocarditis respectively by PCR analysis.Of the 20 dental caries isolates of E. faecalis, 14/20 (70%) and 8/20 (40%) of them were found to be positive for hemolysin and gelatinase activities respectively. Among the 20 E. faecalisstrains, 4 (20%) of them found to have efaA gene encoding for endocarditis, whereas none of the isolates showed positive for ace gene encoding for collagen binding protein. However, 12/20 (60%) of them were harboring both ace and efaA genes together. Our study showed that most of our strains produced hemolysin followed by gealtinase and they found to be sensitive to most of the commonly used antibiotics. Many were equally have the ability to exhibit both collagen binding protein and endocaridtis.
Authors and Affiliations
Dr. S. Arun Kumar BDS, Dr. Ajitha. P MDS, Dr. Gopinath Prakasam Ph. D
Keywords
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- EP ID EP428293
- DOI 10.9790/0853-1712086771.
- Views 64
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