Genotypic Characterization of Drug Resistant Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria in Clinical Isolates from Subjects Resident in Rivers State, Nigeria
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 12
Abstract
The emergence and increasing prevalence of Mycobacterium tuberculosis strains resistant to first and second line anti-tuberculous medications are exacerbating the global TB epidemic. The aim of this study was to genotypically characterize and identify resistant genes of Mycobacterium tuberculosis and nonmycobacterium tuberculosis isolates recovered from clinical specimens of patients infected with Drug Resistant Tuberculosis strains and test the isolates against first and second line anti tuberculosis drugs. Three hundred and ninety (390) sputum samples were collected from the study participants in University of Port Harcourt Teaching Hospital, Braithwaite Memorial Hospital, Bori, Ahoada, Degema and Chest Clinic Rivers State Port Harcourt. The samples were received and analyzed by decontaminating with NaOH-Citrate NaCl method before conventionally inoculating them unto Lowenstein Jensen slants and incubated at 37ᵒC for 8weeks. GeneXpert was analysed by decontaminating with isopropanol under a biosafety cabinet which were later processed in the GeneXpert machine. DNA was extracted with the chemical method using genolyse following manufacturer’s instructions. First line drug susceptibility testing was done genotypically with Genotype MTBDRplus. Second line Drug susceptibility testing was done genotypically with Genotype MTBDRsl kit and sequencing was done with 16S rNA sequence analysis. Total number of participants was higher in females (61.53%) than in males (38.46%) p<0.05 and there was no significant statistical difference in prevalence of TB with respect to site. Prevalence of MTB-NTM co-infection was 14.10%. Of the 390 samples tested, 45 (11.53%) were positive for MTB out of which 10(2.56%) were resistant for rifampicin. 52(13.33%) detected MTB out of which 14(3.58%) were resistant for rifampicin respectively. LPA and culture was also compared, of the 390 samples, 45(11.3%) were positive for MTB out of which 10(2.56%) were rifampicin resistant for LPA and 39(10.00%) were culture positive out of which 9(2.30) were rifampicin resistant. Resistance due to isoniazid was 6(1.53%) for LPA and 4(1.02%) for culture. Multiple resistance for rifampicin and isoniazide was 16(4.10) for LPA and 13(3.33) for culture. The same was done for GeneXpert and Culture. Xpert detected 52(13.33%) MTB out of which 14(3.58%) were rifampicin resistant and Culture isolated 39(10.00%) MTB out of which 9(2.30%) were rifampicin resistant. There was no statistical significance observed with respect to type of www.jmscr.igmpublication.org Impact Factor (SJIF): 6.379 Index Copernicus Value: 79.54 ISSN (e)-2347-176x ISSN (p) 2455-0450 DOI: https://dx.doi.org/10.18535/jmscr/v6i12.41 Mac-Fiberesima Gborieneomie et al JMSCR Volume 06 Issue 12 December 2018 Page 264 JMSCR Vol||06||Issue||12||Page 263-274||December 2018 assay. 57(14.61%) were tested against second line LPA out of which 4(7.01%) were resistant to the fluoroquinolones and none was resistant to the aminoglycosides which was predominant among age 21-40 years. Seven out of the sixteen isolates were sequenced. Samples 1, 3, 4, 6 and 8 detected amplification for rpoB gene responsible for rifampicin resistance (57.14%) and samples 3, 4, and 8 (42.85%) detected amplification for katG gene. Samples 8, 9, 10, 11, and 12 were NTMs which were detected also for rpoB (100%) and sample 10 (20%) alone detected amplification for katG gene. No resistant gene was detected for inhA. 5(31.25%) NTM from the 16NMTBC isolated from the study were sequenced. Mycobacterium abscessus (40%), Mycobacterium setense (20%), Mycobacterium peregrinum (20%) and Mycobacterium conceptionense (20%). Pragmatic efforts are needed to halt drug resistance from progressing beyond what has been identified. It is recommended that people develop the habit of accessing health care early enough to stop further progress in TB.
Authors and Affiliations
Mac-Fiberesima Gborieneomie
Keywords
Related Articles
- EP ID EP501099
- DOI -
- Views 75
- Downloads 0
Factors Determining Outcome in Multivisceral Resection for Gastric Cancer – A South Indian Tertiary Care Centre Observational Study
Gastric cancer is one of the leading cause of cancer related deaths. In India, Chennai, has a very high incidence of 11.8/100000 among males and 5.9/100000 among females. The resectability rate for gastric cancer is abou...
Antihyperlipidaemia Effect of Almond Nuts (Prunus Amygdalus) on Diabetic Rats
diabetic rats. Sixty male albino rats were divided into six groups (A-F) representing the positive and negative controls, and the test groups for four weeks were used. Diabetes were induced in groups C, D, E and F, excep...
Early Detection of Cirrhotic Cardiomyopathy Using Cardiac Bio-Markers: A Cross Sectional Study
Introduction: Cirrhosis is well known to be associated with severe hemodynamic changes like hyperdynamic circulation, increased cardiac output, increased heart rate and decreased systemic vascular resistance resulting in...
Indications and Patterns of Upper Gastro Intestinal Endoscopy Findings among Patients Attending A Tertiary Care Hospital, Kolkata, India
Introduction: Upper Gastrointestinal (UGI) endoscopy has become part and parcel of investigation protocol in a patient with gastrointestinal diseases. With passage of time it has been transformed to a therapeutic modalit...
Evaluation of Effects of Hypo/Hyperthyroidism on Glycated Haemoglobin in Euglycaemic Patients
American Diabetes Association has suggested the use of HbA1c as diagnostic tool for prediabetes and diabetes. A value between 5.7% and 6.5% represents prediabetes while a value ≥6.5% is considered as diabetes mellitus. H...