Genotyping of news variants of the avian infectious bronchitis virus from Tolima department, Colombia
Journal Title: Revista MVZ Cordoba - Year 2016, Vol 21, Issue 3
Abstract
Objective. The aim of this study was identify the different genotypes of infectious bronchitis virus (IVB) present in commercial poultry farms from different localities of the Tolima Department, Colombia. Materials and methods. 105 samples of tracheal swabs of poultry of 21 farms were collected. Poultry had been vaccinated against IVB. An screen to identify positive samples and posteriorly the sequencing of the partial region of the S1 subunit and phylogenetic analysis of the isolates with the reference strains, including the vaccine currently used in the country was performed. Results. Poultry all farms had respiratory signs, but only four farms was confirmed the disease. Positive samples of the IBV (HT6, HT9, HT10 and HT11) were pathogenic for embryos 9-days-old. The HT6 sample was grouped in the same cluster that the Massachusetts strains. The HT9 and HT11 samples showed 99% similarity and were grouped genetically distant from the reference strains and other isolated. The HT10 sample showed low similarities with the isolates and reference strains, grouping alone in another cluster. Conclusions. New genotypes are circulating in the Tolima Department, where there is a risk of genetic recombination. Is believed that vaccines used were not providing cross-protection against the new genotypes.
Authors and Affiliations
Analorena Cifuentes-Rincón, MVZ *| Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, São Paulo, Brasil.Correspondence: lorenacifuentesmvz@gmail.com, Priscila D Lopes, M. Sc| Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, São Paulo, Brasil., Rosa A Sanmiguel P, M. Sc| Universidad Cooperativa de Colombia, Facultad de Medicina Veterinaria y Zootecnia, Laboratorio de morfofisiología, grupo IMPRONTA, Sede Ibagué - Colombia.
Keywords
Related Articles
- EP ID EP3534
- DOI -
- Views 461
- Downloads 25
Chemical and organoleptic evaluation of maralfalfa silage (Pennisetum sp.) plus fresh cassava (Manihot esculenta)
Objective. To evaluate the chemical composition and the organoleptic traits of maralfalfa silage (Pennisetum sp.), containing different proportions of fresh cassava (Manihot esculenta). Materials and methods. Four treatm...
Modeling the lactation curves for milk, fat and protein yield of Holstein cattle in Antioquia, Colombia
Objective. Modeling lactation curves for milk yield and for fat (%) and protein (%) and to determine the main factors affecting the lactation curve. Materials and methods. Official milk control data of 1532 Holstein dair...
Characterization and study of spontaneous fermentation of fermented milk product (suero costeño) produced in Monteria
Objective. Describe the fermentation process of fermented milk product (suero costeño) produced in Monteria. Materials and methods. The fermented homemade product was characterized by physic-chemical and bromatological m...
Hematological and clinical chemistry changes induced by acute stress during handling and capture of catfish (Ictalurus punctatus)
Evaluation of hematological and biochemical parameters of culture channel catfish (Ictalurus punctatus) under acute stress by management and capture practice.Fish (200 g mean) were maintained in culture tanks and divided...
Lipofundin 20% induces hepatic lipid peroxidation in New Zealand white rabbits
Objective. The aim of the present work was to evaluate the effects of Lipofundin 20% on lipid peroxidation markers in the liver of New Zealand white rabbits. Materials and methods. The animals were treated with an intrav...