GROWTH AND DEVELOPMENT OF ROOTED PLANTLETS OF BACOPA MONNIERI L. IN AUXIN-FREE TISSUE CULTURE MEDIUM

Journal Title: International Journal of Botany and Research (IJBR) - Year 2017, Vol 7, Issue 3

Abstract

Indefinite number of platelets of Bacopa monnieri were regenerated from field grown shoot apex explants when implanted on auxin-free Murashige and Skoog (MS) medium supplemented with different cytokines (BA or KN), growth adjuvant adenine sulfate (AdS) and a small amount of citric acid. It was observed that when BA (or BAP) alone was used for multiplication of shoot apex/ bud explants, the proliferated shoots exhibited slower and stunted growth accompanied with abscission of leaves and shoot tip necrosis (STN). The problem of leaf abscission and STN was considerably reduced by the application of AdS and citric acid in the BA (or BAP) supplemented medium which led to the production of maximum 10 platelets with mean shoot length of 8.5 cm per culture within two months. There were no visible morphological abnormalities observed in the micropropagated plantlets. The formation of abnormal callus-like growth or tumors or tissue proliferation at the base of the elongated stem was observed which affected rooting competence of the elongated shoots. Tissue culture is an extraordinary stressful treatment of the plant tissue, which induces the production of phenolic compounds in the medium. It seems the root stimulating efficiency of various phenolic compounds and their relationship with auxins which enhance rooting even in auxin-free medium. Two and half months old in vitro grown plantlets were transferred to polyglasses containing sterile coco peat for acclimatization. Survival rate was observed 100% when acclimatized plantlets were transferred to soil.

Authors and Affiliations

ANITA MEHTA

Keywords

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  • EP ID EP223372
  • DOI 10.24247/ijbrjun20172
  • Views 163
  • Downloads 0

How To Cite

ANITA MEHTA (2017). GROWTH AND DEVELOPMENT OF ROOTED PLANTLETS OF BACOPA MONNIERI L. IN AUXIN-FREE TISSUE CULTURE MEDIUM. International Journal of Botany and Research (IJBR), 7(3), 11-16. https://europub.co.uk/articles/-A-223372