GROWTH KINETICS OF Bacillus subtilis IN LIGNOCELLULOSIC CARBON SOURCES
Journal Title: International Journal of Microbiology Research - Year 2014, Vol 6, Issue 2
Abstract
In this study, the growth kinetics of Bacillus subtilis in M9 minimal medium containing lignocellulosic components such as cellulose, lignin and D-xylose as a sole carbon source were determined. Cell growth in glucose, sucrose and starch were also studied. Studies were conducted in a 2.0 liter fermenter. The specific growth rates and maximum specific growth rates of B. subtilis in 0.1 % of the carbon sources mentioned were determined. The substrate utilization constant of B. subtilis was determined using a wide range of concentrations. The change in pO2, acid and alkali additions during the growth in the fermenter was recorded. The study revealed that B. subtilis was grown in all of the carbon sources tested. The specific growth rate (h-1), maximum specific growth rate (h-1) and substrate utilization constant (mg/L) of B. subtilis in minimal medium containing glucose, sucrose, starch, cellulose, xylose and lignin were (0.481, 0.2, 1.3), (0.334, 0.1, 39.58), (0.074, 0.094, 67.32), (0.046, 0.049, 500), (0.077, 0.136, 132.2) and (0.034, 0.07,660) respectively. In all the carbon sources except cellulose, a decrease in pH was observed. The study concludes that B. subtilis can be efficiently grown using minimal medium containing lignocellulosic substrates as a sole carbon source.
Authors and Affiliations
V. MAGESHWARAN, F. III. INMANN, L. D. HOLMES
Keywords
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