ISOLATION AND CHARACTERIZATION OF THERMOPHILIC ACTINOMYCETES WITH EXTRACELLULAR ENZYME AND BIO-SURFACTANT PRODUCTION POTENTIAL FROM THAR DESERT, INDIA
Journal Title: International Journal of Microbiology Research - Year 2016, Vol 8, Issue 4
Abstract
In the present study thermophilic actinomycetes isolates were isolated from Thar desert of India. Identification of four actinomycetes isolates were done by various morphological and biochemical tests. All the isolates were screened for the presence of extracellular enzyme and bio-surfactant production potential. The results showed that the isolates namely TDI-7, TDI-10, TDI-12, TDI-13 showed the presence of extracellular enzymes and bio-surfactant activity. Isolate TDI-7 and TDI-12 showed the presence of protease production whereas in TDI-10, TDI-12, TDI-13 amylase activity was reported. Lipase activity was found absent in all the isolates. Significant bio-surfactant activity was reported against TDI-13. Further molecular identification of these four isolates were done by partial 16S rRNA gene sequence analysis which showed 95-99 % similarities of references sequences TDI-7 with Streptomyces sp., TDI-10 is with Streptomyces sp., TDI-12 with Streptomyces variabilis strain and TDI-13 with Streptomyces rubiginosus during NCBI blast.
Authors and Affiliations
RUCHIKA SHARMA
Keywords
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